Modifications to improve the effectiveness of restriction fragment length polymorphism typing.

Bruce Budowle, F. S. Baechtel

Research output: Contribution to journalArticle

129 Scopus citations

Abstract

A streamlined and effective method for RFLP analysis of DNA has been developed. Southern transfers are accomplished by alkali blotting DNA onto positively charged nylon membranes. The prehybridization step has been eliminated. The hybridization solution is composed of three cost-effective reagents: 7% SDS, 10% PEG, and phosphate buffer. By using probes that hybridize to variable number of tandem repeat loci, and RFLP analysis of one to two micrograms of genomic DNA can be achieved within five working days under normal working hours. With longer autoradiographic exposures, as little as 20-100 ng of human genomic DNA is sufficient for analysis.

Original languageEnglish
Pages (from-to)181-187
Number of pages7
JournalApplied and theoretical electrophoresis : the official journal of the International Electrophoresis Society
Volume1
Issue number4
StatePublished - 1 Jan 1990

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