MIEN1 drives breast tumor cell migration by regulating cytoskeletal-focal adhesion dynamics

Marilyne Kpetemey, Pankaj Chaudhary, Timothy V. Van Treuren, Jamboor K. Vishwanatha

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Migration and invasion enhancer 1 (MIEN1) is an important regulator of cell migration and invasion. MIEN1 overexpression represents an oncogenic event that promotes tumor cell dissemination and metastasis. The underlying mechanism by which MIEN1 regulates migration and invasion has yet to be deciphered. Here, we demonstrate that MIEN1 acts as a cytoskeletal-signaling adapter protein to drive breast cancer cell migration. MIEN1 localization is concentrated underneath the actin-enriched protrusive structures of the migrating breast cancer cells. Depletion of MIEN1 led to the loss of actin-protrusive structures whereas the over-expression of MIEN1 resulted in rich and thick membrane extensions. Knockdown of MIEN1 also decreased the cellsubstratum adhesion, suggesting a role for MIEN1 in actin cytoskeletal dynamics. Our results show that MIEN1 supports the transition of G-actin to F-actin polymerization and stabilizes F-actin polymers. Additionally, MIEN1 promotes cellular adhesion and actin dynamics by inducing phosphorylation of FAK at Tyr-925 and reducing phosphorylation of cofilin at Ser-3, which results in breast cancer cell migration. Collectively, our data show that MIEN1 plays an essential role in maintaining the plasticity of the dynamic membrane-associated actin cytoskeleton, which leads to an increase in cell motility. Hence, targeting MIEN1 might represent a promising means to prevent breast tumor metastasis.

Original languageEnglish
Pages (from-to)54913-54924
Number of pages12
JournalOncotarget
Volume7
Issue number34
DOIs
StatePublished - 1 Jan 2016

Fingerprint

Focal Adhesions
Cell Movement
Actins
Breast Neoplasms
Phosphorylation
Actin Depolymerizing Factors
Neoplasm Metastasis
Membranes
Actin Cytoskeleton
Polymerization
Polymers

Keywords

  • Actin polymerization
  • Cofilin
  • Focal adhesion kinase
  • MIEN1
  • Migration

Cite this

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title = "MIEN1 drives breast tumor cell migration by regulating cytoskeletal-focal adhesion dynamics",
abstract = "Migration and invasion enhancer 1 (MIEN1) is an important regulator of cell migration and invasion. MIEN1 overexpression represents an oncogenic event that promotes tumor cell dissemination and metastasis. The underlying mechanism by which MIEN1 regulates migration and invasion has yet to be deciphered. Here, we demonstrate that MIEN1 acts as a cytoskeletal-signaling adapter protein to drive breast cancer cell migration. MIEN1 localization is concentrated underneath the actin-enriched protrusive structures of the migrating breast cancer cells. Depletion of MIEN1 led to the loss of actin-protrusive structures whereas the over-expression of MIEN1 resulted in rich and thick membrane extensions. Knockdown of MIEN1 also decreased the cellsubstratum adhesion, suggesting a role for MIEN1 in actin cytoskeletal dynamics. Our results show that MIEN1 supports the transition of G-actin to F-actin polymerization and stabilizes F-actin polymers. Additionally, MIEN1 promotes cellular adhesion and actin dynamics by inducing phosphorylation of FAK at Tyr-925 and reducing phosphorylation of cofilin at Ser-3, which results in breast cancer cell migration. Collectively, our data show that MIEN1 plays an essential role in maintaining the plasticity of the dynamic membrane-associated actin cytoskeleton, which leads to an increase in cell motility. Hence, targeting MIEN1 might represent a promising means to prevent breast tumor metastasis.",
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MIEN1 drives breast tumor cell migration by regulating cytoskeletal-focal adhesion dynamics. / Kpetemey, Marilyne; Chaudhary, Pankaj; Van Treuren, Timothy V.; Vishwanatha, Jamboor K.

In: Oncotarget, Vol. 7, No. 34, 01.01.2016, p. 54913-54924.

Research output: Contribution to journalArticleResearchpeer-review

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