Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant

Rosane Silva, Darcy Muniz De Almeida, Bianca Catarina Azeredo Cabral, Victor Hugo Giordano Dias, Isadora Cristina De Toledo E Mello, Turán Péterürményi, August Eric Woerner, Rodrigo Soares De Moura Neto, Bruce Budowle, Cristina Aparecida Gomes Nassar

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Microorganisms developing in the liner of the spent fuel pool (SFP) and the fuel transfer channel (FTC) of a Nuclear Power Plant (NPP) can form high radiation resistant biofilms and cause corrosion. Due to difficulties and limitations to obtain large samples from SFP and FTC, cotton swabs were used to collect the biofilm from the wall of these installations. Molecular characterization was performed using massively parallel sequencing to obtain a taxonomic and functional gene classification. Also, samples from the drainage system were evaluated because microorganisms may travel over the 12-meter column of the pool water of the Brazilian Nuclear Power Plant (Angra1), which has been functioning since 1985. Regardless of the treatment of the pool water, our data reveal the unexpected presence of Fungi (Basidiomycota and Ascomycota) as the main contaminators of the SFP and FTC. Ustilaginomycetes (Basidiomycota) was the major class contributor (70%) in the SFP and FTC reflecting the little diversity in these sites; nevertheless, Proteobacteria, Actinobacteria, Firmicutes (Bacilli) were present in small proportions. Mapping total reads against six fungal reference genomes indicate that there is, in fact, a high abundance of fungal sequences in samples collected from SFP and FTC. Analysis of the ribosomal internal transcribed spacer (ITS) 1 and 2 regions and the protein found in the mitochondria of eukaryotic cells, cytochrome b (cytb) grouped our sample fungi in the clade 7 as Ustilago and Pseudozyma. In contrast, in the drainage system, Alphaproteobacteria were present in high abundances (55%). The presence of Sphingopyxis, Mesorhizobium, Erythrobacter, Sphingomonas, Novosphingobium, Sphingobium, Chelativorans, Oceanicaulis, Acidovorax, and Cyanobacteria was observed. Based on genomic annotation data, the assessment of the biological function found a higher proportion of protein-coding sequences related to respiration and protein metabolism in SFP and FTC samples. The knowledge of this biological inventorypresent in the system may contribute to further studies of potential microorganisms that might be useful for bioremediation of nuclear waste.

Original languageEnglish
Article numbere0205228
JournalPLoS ONE
Volume13
Issue number10
DOIs
StatePublished - 1 Oct 2018

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Microbial Genes
Nuclear Power Plants
nuclear power
Spent fuels
power plants
Nuclear power plants
Basidiomycota
Genes
Biofilms
Drainage
Sphingomonadaceae
Fungi
Comamonadaceae
Fungal Genome
Mesorhizobium
Ustilago
Sphingomonas
Microorganisms
Radioactive Waste
Alphaproteobacteria

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Silva, R., De Almeida, D. M., Cabral, B. C. A., Dias, V. H. G., De Toledo E Mello, I. C., Péterürményi, T., ... Nassar, C. A. G. (2018). Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant. PLoS ONE, 13(10), [e0205228]. https://doi.org/10.1371/journal.pone.0205228
Silva, Rosane ; De Almeida, Darcy Muniz ; Cabral, Bianca Catarina Azeredo ; Dias, Victor Hugo Giordano ; De Toledo E Mello, Isadora Cristina ; Péterürményi, Turán ; Woerner, August Eric ; De Moura Neto, Rodrigo Soares ; Budowle, Bruce ; Nassar, Cristina Aparecida Gomes. / Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant. In: PLoS ONE. 2018 ; Vol. 13, No. 10.
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abstract = "Microorganisms developing in the liner of the spent fuel pool (SFP) and the fuel transfer channel (FTC) of a Nuclear Power Plant (NPP) can form high radiation resistant biofilms and cause corrosion. Due to difficulties and limitations to obtain large samples from SFP and FTC, cotton swabs were used to collect the biofilm from the wall of these installations. Molecular characterization was performed using massively parallel sequencing to obtain a taxonomic and functional gene classification. Also, samples from the drainage system were evaluated because microorganisms may travel over the 12-meter column of the pool water of the Brazilian Nuclear Power Plant (Angra1), which has been functioning since 1985. Regardless of the treatment of the pool water, our data reveal the unexpected presence of Fungi (Basidiomycota and Ascomycota) as the main contaminators of the SFP and FTC. Ustilaginomycetes (Basidiomycota) was the major class contributor (70{\%}) in the SFP and FTC reflecting the little diversity in these sites; nevertheless, Proteobacteria, Actinobacteria, Firmicutes (Bacilli) were present in small proportions. Mapping total reads against six fungal reference genomes indicate that there is, in fact, a high abundance of fungal sequences in samples collected from SFP and FTC. Analysis of the ribosomal internal transcribed spacer (ITS) 1 and 2 regions and the protein found in the mitochondria of eukaryotic cells, cytochrome b (cytb) grouped our sample fungi in the clade 7 as Ustilago and Pseudozyma. In contrast, in the drainage system, Alphaproteobacteria were present in high abundances (55{\%}). The presence of Sphingopyxis, Mesorhizobium, Erythrobacter, Sphingomonas, Novosphingobium, Sphingobium, Chelativorans, Oceanicaulis, Acidovorax, and Cyanobacteria was observed. Based on genomic annotation data, the assessment of the biological function found a higher proportion of protein-coding sequences related to respiration and protein metabolism in SFP and FTC samples. The knowledge of this biological inventorypresent in the system may contribute to further studies of potential microorganisms that might be useful for bioremediation of nuclear waste.",
author = "Rosane Silva and {De Almeida}, {Darcy Muniz} and Cabral, {Bianca Catarina Azeredo} and Dias, {Victor Hugo Giordano} and {De Toledo E Mello}, {Isadora Cristina} and Tur{\'a}n P{\'e}ter{\"u}rm{\'e}nyi and Woerner, {August Eric} and {De Moura Neto}, {Rodrigo Soares} and Bruce Budowle and Nassar, {Cristina Aparecida Gomes}",
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Silva, R, De Almeida, DM, Cabral, BCA, Dias, VHG, De Toledo E Mello, IC, Péterürményi, T, Woerner, AE, De Moura Neto, RS, Budowle, B & Nassar, CAG 2018, 'Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant', PLoS ONE, vol. 13, no. 10, e0205228. https://doi.org/10.1371/journal.pone.0205228

Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant. / Silva, Rosane; De Almeida, Darcy Muniz; Cabral, Bianca Catarina Azeredo; Dias, Victor Hugo Giordano; De Toledo E Mello, Isadora Cristina; Péterürményi, Turán; Woerner, August Eric; De Moura Neto, Rodrigo Soares; Budowle, Bruce; Nassar, Cristina Aparecida Gomes.

In: PLoS ONE, Vol. 13, No. 10, e0205228, 01.10.2018.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant

AU - Silva, Rosane

AU - De Almeida, Darcy Muniz

AU - Cabral, Bianca Catarina Azeredo

AU - Dias, Victor Hugo Giordano

AU - De Toledo E Mello, Isadora Cristina

AU - Péterürményi, Turán

AU - Woerner, August Eric

AU - De Moura Neto, Rodrigo Soares

AU - Budowle, Bruce

AU - Nassar, Cristina Aparecida Gomes

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AB - Microorganisms developing in the liner of the spent fuel pool (SFP) and the fuel transfer channel (FTC) of a Nuclear Power Plant (NPP) can form high radiation resistant biofilms and cause corrosion. Due to difficulties and limitations to obtain large samples from SFP and FTC, cotton swabs were used to collect the biofilm from the wall of these installations. Molecular characterization was performed using massively parallel sequencing to obtain a taxonomic and functional gene classification. Also, samples from the drainage system were evaluated because microorganisms may travel over the 12-meter column of the pool water of the Brazilian Nuclear Power Plant (Angra1), which has been functioning since 1985. Regardless of the treatment of the pool water, our data reveal the unexpected presence of Fungi (Basidiomycota and Ascomycota) as the main contaminators of the SFP and FTC. Ustilaginomycetes (Basidiomycota) was the major class contributor (70%) in the SFP and FTC reflecting the little diversity in these sites; nevertheless, Proteobacteria, Actinobacteria, Firmicutes (Bacilli) were present in small proportions. Mapping total reads against six fungal reference genomes indicate that there is, in fact, a high abundance of fungal sequences in samples collected from SFP and FTC. Analysis of the ribosomal internal transcribed spacer (ITS) 1 and 2 regions and the protein found in the mitochondria of eukaryotic cells, cytochrome b (cytb) grouped our sample fungi in the clade 7 as Ustilago and Pseudozyma. In contrast, in the drainage system, Alphaproteobacteria were present in high abundances (55%). The presence of Sphingopyxis, Mesorhizobium, Erythrobacter, Sphingomonas, Novosphingobium, Sphingobium, Chelativorans, Oceanicaulis, Acidovorax, and Cyanobacteria was observed. Based on genomic annotation data, the assessment of the biological function found a higher proportion of protein-coding sequences related to respiration and protein metabolism in SFP and FTC samples. The knowledge of this biological inventorypresent in the system may contribute to further studies of potential microorganisms that might be useful for bioremediation of nuclear waste.

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Silva R, De Almeida DM, Cabral BCA, Dias VHG, De Toledo E Mello IC, Péterürményi T et al. Microbial enrichment and gene functional categories revealed on the walls of a spent fuel pool of a nuclear power plant. PLoS ONE. 2018 Oct 1;13(10). e0205228. https://doi.org/10.1371/journal.pone.0205228