Methylene blue protects astrocytes against glucose oxygen deprivation by improving cellular respiration

Gourav Roy Choudhury, Ali Winters, Ryan M. Rich, Myoung Gwi Ryou, Zygmunt Gryczynski, Fang Yuan, Shaohua Yang, Ran Liu

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Astrocytes outnumber neurons and serve many metabolic and trophic functions in the mammalian brain. Preserving astrocytes is critical for normal brain function as well as for protecting the brain against various insults. Our previous studies have indicated that methylene blue (MB) functions as an alternative electron carrier and enhances brain metabolism. In addition, MB has been shown to be protective against neurodegeneration and brain injury. In the current study, we investigated the protective role of MB in astrocytes. Cell viability assays showed that MB treatment significantly protected primary astrocytes from oxygenglucose deprivation (OGD) & reoxygenation induced cell death. We also studied the effect of MB on cellular oxygen and glucose metabolism in primary astrocytes following OGD-reoxygenation injury. MB treatment significantly increased cellular oxygen consumption, glucose uptake and ATP production in primary astrocytes. In conclusion our study demonstrated that MB protects astrocytes against OGD-reoxygenation injury by improving astrocyte cellular respiration.

Original languageEnglish
Article numbere0123096
JournalPLoS ONE
Volume10
Issue number4
DOIs
StatePublished - 7 Apr 2015

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Cell Respiration
methylene blue
Methylene Blue
astrocytes
cell respiration
Astrocytes
Oxygen
oxygen
Glucose
glucose
Brain
brain
Metabolism
metabolism
Wounds and Injuries
Cell death
Oxygen Consumption
Brain Injuries
oxygen consumption
Neurons

Cite this

Choudhury, Gourav Roy ; Winters, Ali ; Rich, Ryan M. ; Ryou, Myoung Gwi ; Gryczynski, Zygmunt ; Yuan, Fang ; Yang, Shaohua ; Liu, Ran. / Methylene blue protects astrocytes against glucose oxygen deprivation by improving cellular respiration. In: PLoS ONE. 2015 ; Vol. 10, No. 4.
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Methylene blue protects astrocytes against glucose oxygen deprivation by improving cellular respiration. / Choudhury, Gourav Roy; Winters, Ali; Rich, Ryan M.; Ryou, Myoung Gwi; Gryczynski, Zygmunt; Yuan, Fang; Yang, Shaohua; Liu, Ran.

In: PLoS ONE, Vol. 10, No. 4, e0123096, 07.04.2015.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Choudhury, Gourav Roy

AU - Winters, Ali

AU - Rich, Ryan M.

AU - Ryou, Myoung Gwi

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AU - Yuan, Fang

AU - Yang, Shaohua

AU - Liu, Ran

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