Methylene blue promotes quiescence of rat neural progenitor cells

Luokun Xie, Gourav R. Choudhury, Jixian Wang, Yong Park, Ran Liu, Fang Yuan, Chun Li Zhang, Thomas Yorio, Kunlin Jin, Shaohua Yang

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

Original languageEnglish
Article number315
JournalFrontiers in Cellular Neuroscience
Volume8
Issue numberOCT
DOIs
StatePublished - 7 Oct 2014

Fingerprint

Methylene Blue
Stem Cells
Intraventricular Infusions
Cyclins
Neural Stem Cells
Cell Aging
Lateral Ventricles
Neurodegenerative Diseases
Down-Regulation
Cell Proliferation
Therapeutics
In Vitro Techniques

Keywords

  • Methylene blue
  • Neural progenitor cell
  • Neurogenesis
  • Proliferation
  • Quiescence

Cite this

Xie, Luokun ; Choudhury, Gourav R. ; Wang, Jixian ; Park, Yong ; Liu, Ran ; Yuan, Fang ; Zhang, Chun Li ; Yorio, Thomas ; Jin, Kunlin ; Yang, Shaohua. / Methylene blue promotes quiescence of rat neural progenitor cells. In: Frontiers in Cellular Neuroscience. 2014 ; Vol. 8, No. OCT.
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Methylene blue promotes quiescence of rat neural progenitor cells. / Xie, Luokun; Choudhury, Gourav R.; Wang, Jixian; Park, Yong; Liu, Ran; Yuan, Fang; Zhang, Chun Li; Yorio, Thomas; Jin, Kunlin; Yang, Shaohua.

In: Frontiers in Cellular Neuroscience, Vol. 8, No. OCT, 315, 07.10.2014.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Methylene blue promotes quiescence of rat neural progenitor cells

AU - Xie, Luokun

AU - Choudhury, Gourav R.

AU - Wang, Jixian

AU - Park, Yong

AU - Liu, Ran

AU - Yuan, Fang

AU - Zhang, Chun Li

AU - Yorio, Thomas

AU - Jin, Kunlin

AU - Yang, Shaohua

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N2 - Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

AB - Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

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