Methylene blue promotes quiescence of rat neural progenitor cells

Luokun Xie, Gourav R. Choudhury, Jixian Wang, Yong Park, Ran Liu, Fang Yuan, Chun Li Zhang, Thomas Yorio, Kunlin Jin, Shao Hua Yang

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4 Scopus citations

Abstract

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

Original languageEnglish
Article number315
JournalFrontiers in Cellular Neuroscience
Volume8
Issue numberOCT
DOIs
Publication statusPublished - 7 Oct 2014

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Keywords

  • Methylene blue
  • Neural progenitor cell
  • Neurogenesis
  • Proliferation
  • Quiescence

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