Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva; Ignacy Gryczynski; Anne Barnett; Zoya Leonenko; Joseph R. Lakowicz; Zygmunt Gryczynski

doi:10.1016/j.ab.2007.01.030

Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

Original language	English
Pages (from-to)	239-245
Number of pages	7
Journal	Analytical Biochemistry
Volume	363
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2007.01.030
State	Published - 15 Apr 2007

Keywords

Fluorescence immunoassay
Metal-enhanced fluorescence
Nano-size metal particles
Plasmonics
Silver island films

Access to Document

10.1016/j.ab.2007.01.030

Cite this

@article{4bc57c2b273f4aa28b7df034bf4486a1,

title = "Metal particle-enhanced fluorescent immunoassays on metal mirrors",

abstract = "We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.",

keywords = "Fluorescence immunoassay, Metal-enhanced fluorescence, Nano-size metal particles, Plasmonics, Silver island films",

author = "Matveeva, {Evgenia G.} and Ignacy Gryczynski and Anne Barnett and Zoya Leonenko and Lakowicz, {Joseph R.} and Zygmunt Gryczynski",

note = "Funding Information: This research was supported by the National Institutes of Health (NCI CA 114460, NIBIB EB-1690, NCRR-08119), BITC, and Philip Morris USA and Philip Morris International.",

year = "2007",

month = apr,

day = "15",

doi = "10.1016/j.ab.2007.01.030",

language = "English",

volume = "363",

pages = "239--245",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Metal particle-enhanced fluorescent immunoassays on metal mirrors

AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Barnett, Anne

AU - Leonenko, Zoya

AU - Lakowicz, Joseph R.

AU - Gryczynski, Zygmunt

N1 - Funding Information: This research was supported by the National Institutes of Health (NCI CA 114460, NIBIB EB-1690, NCRR-08119), BITC, and Philip Morris USA and Philip Morris International.

PY - 2007/4/15

Y1 - 2007/4/15

N2 - We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

AB - We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

KW - Fluorescence immunoassay

KW - Metal-enhanced fluorescence

KW - Nano-size metal particles

KW - Plasmonics

KW - Silver island films

UR - http://www.scopus.com/inward/record.url?scp=33947286758&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2007.01.030

DO - 10.1016/j.ab.2007.01.030

M3 - Article

C2 - 17316540

AN - SCOPUS:33947286758

SN - 0003-2697

VL - 363

SP - 239

EP - 245

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Metal particle-enhanced fluorescent immunoassays on metal mirrors

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this