Metal particle-enhanced fluorescent immunoassays on metal mirrors

Evgenia G. Matveeva, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R. Lakowicz, Zygmunt Gryczynski

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalAnalytical Biochemistry
Volume363
Issue number2
DOIs
StatePublished - 15 Apr 2007

Fingerprint

Immunoassay
Silver
Islands
Metals
Glass
Fluoroimmunoassay
Metal Nanoparticles
Myoglobin
Metal nanoparticles
Aluminum
Silicon Dioxide
Gold
Assays
Mirrors
Immunoglobulin G
Rabbits
Antibodies

Keywords

  • Fluorescence immunoassay
  • Metal-enhanced fluorescence
  • Nano-size metal particles
  • Plasmonics
  • Silver island films

Cite this

Matveeva, Evgenia G. ; Gryczynski, Ignacy ; Barnett, Anne ; Leonenko, Zoya ; Lakowicz, Joseph R. ; Gryczynski, Zygmunt. / Metal particle-enhanced fluorescent immunoassays on metal mirrors. In: Analytical Biochemistry. 2007 ; Vol. 363, No. 2. pp. 239-245.
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Metal particle-enhanced fluorescent immunoassays on metal mirrors. / Matveeva, Evgenia G.; Gryczynski, Ignacy; Barnett, Anne; Leonenko, Zoya; Lakowicz, Joseph R.; Gryczynski, Zygmunt.

In: Analytical Biochemistry, Vol. 363, No. 2, 15.04.2007, p. 239-245.

Research output: Contribution to journalArticle

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AU - Matveeva, Evgenia G.

AU - Gryczynski, Ignacy

AU - Barnett, Anne

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AU - Gryczynski, Zygmunt

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