Abstract
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.
Original language | English |
---|---|
Pages (from-to) | 239-245 |
Number of pages | 7 |
Journal | Analytical Biochemistry |
Volume | 363 |
Issue number | 2 |
DOIs | |
State | Published - 15 Apr 2007 |
Keywords
- Fluorescence immunoassay
- Metal-enhanced fluorescence
- Nano-size metal particles
- Plasmonics
- Silver island films