Metal-enhanced immunoassays

Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko, Pabak Sarkar, Julian Borejdo, Irina Akopova, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages217-229
Number of pages13
ISBN (Print)9781617798054
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

Keywords

  • Alexa488
  • Fluorescence enhancement
  • Immunoassay
  • Plasmonics
  • Silver nanoparticles

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    Gryczynski, I., Luchowski, R., Matveeva, E. G., Shtoyko, T., Sarkar, P., Borejdo, J., Akopova, I., & Gryczynski, Z. (2012). Metal-enhanced immunoassays. In Spectroscopic Methods of Analysis: Methods and Protocols (pp. 217-229). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_10