Abstract
The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.
| Original language | English |
|---|---|
| Title of host publication | Spectroscopic Methods of Analysis |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 217-229 |
| Number of pages | 13 |
| ISBN (Print) | 9781617798054 |
| DOIs | |
| State | Published - 1 Jan 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 875 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Alexa488
- Fluorescence enhancement
- Immunoassay
- Plasmonics
- Silver nanoparticles
Cite this
}
Metal-enhanced immunoassays. / Gryczynski, Ignacy; Luchowski, Rafal; Matveeva, Evgenia G.; Shtoyko, Tanya; Sarkar, Pabak; Borejdo, Julian; Akopova, Irina; Gryczynski, Zygmunt.
Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. p. 217-229 (Methods in Molecular Biology; Vol. 875).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - Metal-enhanced immunoassays
AU - Gryczynski, Ignacy
AU - Luchowski, Rafal
AU - Matveeva, Evgenia G.
AU - Shtoyko, Tanya
AU - Sarkar, Pabak
AU - Borejdo, Julian
AU - Akopova, Irina
AU - Gryczynski, Zygmunt
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.
AB - The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.
KW - Alexa488
KW - Fluorescence enhancement
KW - Immunoassay
KW - Plasmonics
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84865808370&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-806-1_10
DO - 10.1007/978-1-61779-806-1_10
M3 - Chapter
C2 - 22573442
AN - SCOPUS:84865808370
SN - 9781617798054
T3 - Methods in Molecular Biology
SP - 217
EP - 229
BT - Spectroscopic Methods of Analysis
PB - Humana Press Inc.
ER -