Metal-enhanced immunoassays

Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko, Pabak Sarkar, Julian Borejdo, Irina Akopova, Zygmunt Gryczynski

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages217-229
Number of pages13
ISBN (Print)9781617798054
DOIs
StatePublished - 1 Jan 2012

Publication series

NameMethods in Molecular Biology
Volume875
ISSN (Print)1064-3745

Fingerprint

Immunoassay
Fluorescence
Metals
Silver
Nanostructures
Fluorescein
Islands
Artifacts
Glass
Polymers
Coloring Agents

Keywords

  • Alexa488
  • Fluorescence enhancement
  • Immunoassay
  • Plasmonics
  • Silver nanoparticles

Cite this

Gryczynski, I., Luchowski, R., Matveeva, E. G., Shtoyko, T., Sarkar, P., Borejdo, J., ... Gryczynski, Z. (2012). Metal-enhanced immunoassays. In Spectroscopic Methods of Analysis: Methods and Protocols (pp. 217-229). (Methods in Molecular Biology; Vol. 875). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-806-1_10
Gryczynski, Ignacy ; Luchowski, Rafal ; Matveeva, Evgenia G. ; Shtoyko, Tanya ; Sarkar, Pabak ; Borejdo, Julian ; Akopova, Irina ; Gryczynski, Zygmunt. / Metal-enhanced immunoassays. Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. pp. 217-229 (Methods in Molecular Biology).
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Gryczynski, I, Luchowski, R, Matveeva, EG, Shtoyko, T, Sarkar, P, Borejdo, J, Akopova, I & Gryczynski, Z 2012, Metal-enhanced immunoassays. in Spectroscopic Methods of Analysis: Methods and Protocols. Methods in Molecular Biology, vol. 875, Humana Press Inc., pp. 217-229. https://doi.org/10.1007/978-1-61779-806-1_10

Metal-enhanced immunoassays. / Gryczynski, Ignacy; Luchowski, Rafal; Matveeva, Evgenia G.; Shtoyko, Tanya; Sarkar, Pabak; Borejdo, Julian; Akopova, Irina; Gryczynski, Zygmunt.

Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc., 2012. p. 217-229 (Methods in Molecular Biology; Vol. 875).

Research output: Chapter in Book/Report/Conference proceedingChapter

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T1 - Metal-enhanced immunoassays

AU - Gryczynski, Ignacy

AU - Luchowski, Rafal

AU - Matveeva, Evgenia G.

AU - Shtoyko, Tanya

AU - Sarkar, Pabak

AU - Borejdo, Julian

AU - Akopova, Irina

AU - Gryczynski, Zygmunt

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.

AB - The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.

KW - Alexa488

KW - Fluorescence enhancement

KW - Immunoassay

KW - Plasmonics

KW - Silver nanoparticles

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BT - Spectroscopic Methods of Analysis

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Gryczynski I, Luchowski R, Matveeva EG, Shtoyko T, Sarkar P, Borejdo J et al. Metal-enhanced immunoassays. In Spectroscopic Methods of Analysis: Methods and Protocols. Humana Press Inc. 2012. p. 217-229. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-806-1_10