@inbook{4d117746217b43ffa3739e903d7edba3,
title = "Metal-enhanced immunoassays",
abstract = "The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monolayers of dyes requires a strong excitation and good detection system. Such samples are susceptible to artifacts due to background fluorescence from substrates. We demonstrate that using silver nanostructures deposited on the slide substrate can significantly enhance measured fluorescence, reduce unwanted background and increase photostability of the used probes. Using thin layers of polymer doped with fluorescein, we tested two nanostructures - silver island films (SIFs) deposited on glass slides and self-assembled colloidal structures (SACS) deposited on thin silver film. The SACS surfaces show extraordinary fluorescence enhancements: over 100-folds in hot spots. We applied these surfaces for enhanced Alexa488 model immunoassay.",
keywords = "Alexa488, Fluorescence enhancement, Immunoassay, Plasmonics, Silver nanoparticles",
author = "Ignacy Gryczynski and Rafal Luchowski and Matveeva, {Evgenia G.} and Tanya Shtoyko and Pabak Sarkar and Julian Borejdo and Irina Akopova and Zygmunt Gryczynski",
year = "2012",
doi = "10.1007/978-1-61779-806-1_10",
language = "English",
isbn = "9781617798054",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "217--229",
booktitle = "Spectroscopic Methods of Analysis",
}