Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva; Zygmunt Gryczynski; Joanna Malicka; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.530091

Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journal › Conference article › peer-review

7 Scopus citations

Abstract

In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

Original language	English
Pages (from-to)	45-53
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5327
DOIs	https://doi.org/10.1117/12.530091
State	Published - 2004
Event	Plasmonics in Biology and Medicine - San Jose, CA, United States Duration: 26 Jan 2004 → 27 Jan 2004

Keywords

Cardiac markers
Enhanced fluorescence
Fluorescence immunoassay
Nano-size metallic particles
Silver islands

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10.1117/12.530091

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title = "Metal-enhanced fluorescent biomarkers for cardiac risk assessment",

abstract = "In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.",

keywords = "Cardiac markers, Enhanced fluorescence, Fluorescence immunoassay, Nano-size metallic particles, Silver islands",

author = "Evgenia Matveeva and Zygmunt Gryczynski and Joanna Malicka and Ignacy Gryczynski and Lakowicz, {Joseph R.}",

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TY - JOUR

T1 - Metal-enhanced fluorescent biomarkers for cardiac risk assessment

AU - Matveeva, Evgenia

AU - Gryczynski, Zygmunt

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

PY - 2004

Y1 - 2004

N2 - In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

AB - In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

KW - Cardiac markers

KW - Enhanced fluorescence

KW - Fluorescence immunoassay

KW - Nano-size metallic particles

KW - Silver islands

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U2 - 10.1117/12.530091

DO - 10.1117/12.530091

M3 - Conference article

AN - SCOPUS:7644242472

SN - 0277-786X

VL - 5327

SP - 45

EP - 53

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

Y2 - 26 January 2004 through 27 January 2004

ER -

Metal-enhanced fluorescent biomarkers for cardiac risk assessment

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Keywords

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