Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalConference article

7 Scopus citations

Abstract

In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5327
DOIs
StatePublished - 2004
EventPlasmonics in Biology and Medicine - San Jose, CA, United States
Duration: 26 Jan 200427 Jan 2004

Keywords

  • Cardiac markers
  • Enhanced fluorescence
  • Fluorescence immunoassay
  • Nano-size metallic particles
  • Silver islands

Fingerprint Dive into the research topics of 'Metal-enhanced fluorescent biomarkers for cardiac risk assessment'. Together they form a unique fingerprint.

  • Cite this