Metal-enhanced fluorescent biomarkers for cardiac risk assessment

Evgenia Matveeva, Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Joseph R. Lakowicz

Research output: Contribution to journalConference article

7 Citations (Scopus)

Abstract

In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5327
DOIs
StatePublished - 16 Nov 2004
EventPlasmonics in Biology and Medicine - San Jose, CA, United States
Duration: 26 Jan 200427 Jan 2004

Fingerprint

Immunoassay
immunoassay
risk assessment
biomarkers
Biomarkers
Risk Assessment
Cardiac
Risk assessment
Fluorescence
Metals
Total Internal Reflection
fluorescence
Rejection
Amplification
rejection
serums
markers
metals
blood
Blood

Keywords

  • Cardiac markers
  • Enhanced fluorescence
  • Fluorescence immunoassay
  • Nano-size metallic particles
  • Silver islands

Cite this

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title = "Metal-enhanced fluorescent biomarkers for cardiac risk assessment",
abstract = "In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.",
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Metal-enhanced fluorescent biomarkers for cardiac risk assessment. / Matveeva, Evgenia; Gryczynski, Zygmunt; Malicka, Joanna; Gryczynski, Ignacy; Lakowicz, Joseph R.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5327, 16.11.2004, p. 45-53.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Metal-enhanced fluorescent biomarkers for cardiac risk assessment

AU - Matveeva, Evgenia

AU - Gryczynski, Zygmunt

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Lakowicz, Joseph R.

PY - 2004/11/16

Y1 - 2004/11/16

N2 - In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

AB - In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

KW - Cardiac markers

KW - Enhanced fluorescence

KW - Fluorescence immunoassay

KW - Nano-size metallic particles

KW - Silver islands

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JO - Proceedings of SPIE - The International Society for Optical Engineering

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