TY - JOUR
T1 - Metal-enhanced fluorescence
T2 - An emerging tool in biotechnology
AU - Aslan, Kadir
AU - Gryczynski, Ignacy
AU - Malicka, Joanna
AU - Matveeva, Evgenia
AU - Lakowicz, Joseph R.
AU - Geddes, Chris D.
N1 - Funding Information:
The authors would like to thank the National Institutes of Health for financial support (National Center for Research Resources, RR-08119 and R21GM070929), Philip Morris USA Inc. and Philip Morris International, and the National Institute for Biomedical Imaging and Bioengineering (EB-1690). The authors also wish to thank the Medical Biotechnology Center, University of Maryland Biotechnology Institute for partial salary support to JRL, CDG and IG. Others involved in this work include Zygmunt Gryczynski, Joanna Lukomska, Jian Zhang, Ramachandram Badugu, Slawomir Makowiec, Kazimierz Nowacyzk, Meng Wu and Jun Huang.
PY - 2005/2
Y1 - 2005/2
N2 - Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detection of a fluorophore is usually limited by its quantum yield, autofluorescence of the samples and/or the photostability of the fluorophores; however, there has been a recent explosion in the use of metallic nanostructures to favorably modify the spectral properties of fluorophores and to alleviate some of these fluorophore photophysical constraints. The use of fluorophore-metal interactions has been termed radiative decay engineering, metal-enhanced fluorescence or surface-enhanced fluorescence.
AB - Over the past 15 years, fluorescence has become the dominant detection/sensing technology in medical diagnostics and biotechnology. Although fluorescence is a highly sensitive technique, where single molecules can readily be detected, there is still a drive for reduced detection limits. The detection of a fluorophore is usually limited by its quantum yield, autofluorescence of the samples and/or the photostability of the fluorophores; however, there has been a recent explosion in the use of metallic nanostructures to favorably modify the spectral properties of fluorophores and to alleviate some of these fluorophore photophysical constraints. The use of fluorophore-metal interactions has been termed radiative decay engineering, metal-enhanced fluorescence or surface-enhanced fluorescence.
UR - http://www.scopus.com/inward/record.url?scp=13844281499&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2005.01.001
DO - 10.1016/j.copbio.2005.01.001
M3 - Review article
C2 - 15722016
AN - SCOPUS:13844281499
SN - 0958-1669
VL - 16
SP - 55
EP - 62
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 1 SPEC. ISS.
ER -