Metal-enhanced fluorescence: A novel approach to ultra-sensitive fluorescence sensing assay platforms

Zygmunt Gryczynski, Joanna Malicka, Ignacy Gryczynski, Evgenia Matveeva, Chris D. Geddes, Kadir Aslan, Joseph R. Lakowicz

Research output: Contribution to journalConference article

6 Citations (Scopus)

Abstract

We describe the development of a novel generic approach to fluorescence sensing based on metal-enhanced fluorescence (MEF). This work follows our initial reports of radiative decay engineering (RDE), where we experimentally demonstrated dramatic signal enhancements of fluorophores positioned close to surface-bound silver nanostructures. The attractive changes in spectral properties of fluorophores includes increased rates of excitation, increased quantum yields, decreased fluorescence lifetimes with an increased photostability, and drastically increased rates of multi-photon excitation. In this report we present a new class of fluorescent biomarkers which are strongly enhanced by metallic particles. This has afforded the development of a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform utilizes metal particles deposited on glass/quartz surfaces, covered with sub-nanometer layers of a fluorescent biomarker. As such the fluorescence signal of the composite is strongly enhanced. This readily allows easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens. We also explore different sensing geometries, such as using evanescent wave excitation.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5321
DOIs
StatePublished - 27 Oct 2004
EventProgress in Biomedical Optics and Imaging - Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies - San Jose, CA, United States
Duration: 25 Jan 200427 Jan 2004

Fingerprint

Fluorescence
Assays
Sensing
platforms
Metals
fluorescence
metals
Excitation
Biomarkers
Fluorophores
biomarkers
Fluorescence Lifetime
Evanescent Wave
Silver
Quartz
Nanostructures
Spectral Properties
evanescent waves
wave excitation
metal particles

Cite this

@article{f96ff53c5d3241109441431b0a371098,
title = "Metal-enhanced fluorescence: A novel approach to ultra-sensitive fluorescence sensing assay platforms",
abstract = "We describe the development of a novel generic approach to fluorescence sensing based on metal-enhanced fluorescence (MEF). This work follows our initial reports of radiative decay engineering (RDE), where we experimentally demonstrated dramatic signal enhancements of fluorophores positioned close to surface-bound silver nanostructures. The attractive changes in spectral properties of fluorophores includes increased rates of excitation, increased quantum yields, decreased fluorescence lifetimes with an increased photostability, and drastically increased rates of multi-photon excitation. In this report we present a new class of fluorescent biomarkers which are strongly enhanced by metallic particles. This has afforded the development of a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform utilizes metal particles deposited on glass/quartz surfaces, covered with sub-nanometer layers of a fluorescent biomarker. As such the fluorescence signal of the composite is strongly enhanced. This readily allows easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens. We also explore different sensing geometries, such as using evanescent wave excitation.",
author = "Zygmunt Gryczynski and Joanna Malicka and Ignacy Gryczynski and Evgenia Matveeva and Geddes, {Chris D.} and Kadir Aslan and Lakowicz, {Joseph R.}",
year = "2004",
month = "10",
day = "27",
doi = "10.1117/12.530072",
language = "English",
volume = "5321",
pages = "275--282",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

Metal-enhanced fluorescence : A novel approach to ultra-sensitive fluorescence sensing assay platforms. / Gryczynski, Zygmunt; Malicka, Joanna; Gryczynski, Ignacy; Matveeva, Evgenia; Geddes, Chris D.; Aslan, Kadir; Lakowicz, Joseph R.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5321, 27.10.2004, p. 275-282.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Metal-enhanced fluorescence

T2 - A novel approach to ultra-sensitive fluorescence sensing assay platforms

AU - Gryczynski, Zygmunt

AU - Malicka, Joanna

AU - Gryczynski, Ignacy

AU - Matveeva, Evgenia

AU - Geddes, Chris D.

AU - Aslan, Kadir

AU - Lakowicz, Joseph R.

PY - 2004/10/27

Y1 - 2004/10/27

N2 - We describe the development of a novel generic approach to fluorescence sensing based on metal-enhanced fluorescence (MEF). This work follows our initial reports of radiative decay engineering (RDE), where we experimentally demonstrated dramatic signal enhancements of fluorophores positioned close to surface-bound silver nanostructures. The attractive changes in spectral properties of fluorophores includes increased rates of excitation, increased quantum yields, decreased fluorescence lifetimes with an increased photostability, and drastically increased rates of multi-photon excitation. In this report we present a new class of fluorescent biomarkers which are strongly enhanced by metallic particles. This has afforded the development of a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform utilizes metal particles deposited on glass/quartz surfaces, covered with sub-nanometer layers of a fluorescent biomarker. As such the fluorescence signal of the composite is strongly enhanced. This readily allows easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens. We also explore different sensing geometries, such as using evanescent wave excitation.

AB - We describe the development of a novel generic approach to fluorescence sensing based on metal-enhanced fluorescence (MEF). This work follows our initial reports of radiative decay engineering (RDE), where we experimentally demonstrated dramatic signal enhancements of fluorophores positioned close to surface-bound silver nanostructures. The attractive changes in spectral properties of fluorophores includes increased rates of excitation, increased quantum yields, decreased fluorescence lifetimes with an increased photostability, and drastically increased rates of multi-photon excitation. In this report we present a new class of fluorescent biomarkers which are strongly enhanced by metallic particles. This has afforded the development of a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform utilizes metal particles deposited on glass/quartz surfaces, covered with sub-nanometer layers of a fluorescent biomarker. As such the fluorescence signal of the composite is strongly enhanced. This readily allows easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens. We also explore different sensing geometries, such as using evanescent wave excitation.

UR - http://www.scopus.com/inward/record.url?scp=5644248263&partnerID=8YFLogxK

U2 - 10.1117/12.530072

DO - 10.1117/12.530072

M3 - Conference article

AN - SCOPUS:5644248263

VL - 5321

SP - 275

EP - 282

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -