TY - JOUR
T1 - Mesenchymal stem cell-based therapy in a mouse model of experimental autoimmune encephalomyelitis (EAE)
AU - Bowles, Annie C.
AU - Scruggs, Brittni A.
AU - Bunnell, Bruce A.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2014.
PY - 2014
Y1 - 2014
N2 - Multiple sclerosis (MS) is a common neurodegenerative disease that presents after an auto-reactive immune response against constituents of the central nervous system. Demyelination, infl ammation, and white matter lesions are all hallmarks of this disease. Clinical research supports the use of mesenchymal stem cells (MSCs) as therapy for MS to ameliorate symptoms and pathology. MSCs can be isolated from multiple tissues, including adipose and bone marrow, and are able to migrate to sites of pathology, release antiinfl ammatory factors, and provide immunomodulatory and neuroprotective effects once administered. Numerous studies have demonstrated the benefi cial effects of MSCs in experimental autoimmune encephalomyelitis (EAE), an induced model of MS. EAE can be induced in several species; however, the mouse is commonly used for therapeutic testing. In the following chapter, scientists will be able to learn how to prepare reagents and MSCs (e.g., isolate, culture, and expand) as well as skillfully execute induction of EAE in mice and administer stem cell-based treatments. Standard methods used to evaluate the disease progression and analyze postmortem tissues are also included.
AB - Multiple sclerosis (MS) is a common neurodegenerative disease that presents after an auto-reactive immune response against constituents of the central nervous system. Demyelination, infl ammation, and white matter lesions are all hallmarks of this disease. Clinical research supports the use of mesenchymal stem cells (MSCs) as therapy for MS to ameliorate symptoms and pathology. MSCs can be isolated from multiple tissues, including adipose and bone marrow, and are able to migrate to sites of pathology, release antiinfl ammatory factors, and provide immunomodulatory and neuroprotective effects once administered. Numerous studies have demonstrated the benefi cial effects of MSCs in experimental autoimmune encephalomyelitis (EAE), an induced model of MS. EAE can be induced in several species; however, the mouse is commonly used for therapeutic testing. In the following chapter, scientists will be able to learn how to prepare reagents and MSCs (e.g., isolate, culture, and expand) as well as skillfully execute induction of EAE in mice and administer stem cell-based treatments. Standard methods used to evaluate the disease progression and analyze postmortem tissues are also included.
KW - EAE
KW - Mesenchymal stem cells
KW - Multiple sclerosis
KW - Murine experimental autoimmune encephalomyelitis
KW - Myelin oligodendrocyte glycoprotein
KW - T cellmediated autoimmune disease
UR - http://www.scopus.com/inward/record.url?scp=84945254104&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1453-1_25
DO - 10.1007/978-1-4939-1453-1_25
M3 - Article
C2 - 25173393
AN - SCOPUS:84945254104
VL - 1213
SP - 303
EP - 319
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -