MED31 involved in regulating self-renewal and adipogenesis of human mesenchymal stem cells

Erik P. Beadle, Joseph A. Straub, Bruce A. Bunnell, Jamie J. Newman

Research output: Contribution to journalArticle

Abstract

Regulation of gene expression is critical for the maintenance of cell state and homeostasis. Aberrant regulation of genes can lead to unwanted cell proliferation or misdirected differentiation. Here we investigate the role of MED31, a highly conserved subunit of the Mediator complex, to determine the role this subunit plays in the maintenance of human mesenchymal stem cell (hMSC) state. Using siRNA-mediated knockdown of MED31 we demonstrate a decrease in self-renewal based on cell assays and monitoring of gene expression. In addition, in the absence of MED31, hMSCs also displayed a reduction in adipogenesis as evidenced by diminished lipid vesicle formation and expression of specific adipogenic markers. These data present evidence for a significant role for MED31 in maintaining adult stem cell homeostasis, thereby introducing potential novel targets for future investigation and use in better understanding stem cell behavior and adipogenesis.

Original languageEnglish
Pages (from-to)1545-1550
Number of pages6
JournalMolecular Biology Reports
Volume45
Issue number5
DOIs
StatePublished - 1 Oct 2018

Keywords

  • Adipogenesis
  • Human mesenchymal stem cells
  • Mediator complex
  • Transcription

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