Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon

David H. Warshauer, Carey P. Davis, Cydne Holt, Yonmee Han, Paulina Walichiewicz, Tom Richardson, Kathryn Stephens, Anne Jager, Jonathan King, Bruce Budowle

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The TruSeq™ Forensic Amplicon library preparation protocol, originally designed to attach sequencing adapters to chromatin-bound DNA for chromatin immunoprecipitation sequencing (TruSeq™ ChIP-Seq), was used here to attach adapters directly to amplicons containing markers of forensic interest. In this study, the TruSeq™ Forensic Amplicon library preparation protocol was used to detect 160 single nucleotide polymorphisms (SNPs), including human identification SNPs (iSNPs), ancestry, and phenotypic SNPs (apSNPs) in 12 reference samples. Results were compared with those generated by a second laboratory using the same technique, as well as to those generated by whole genome sequencing (WGS). The genotype calls made using the TruSeq™ Forensic Amplicon library preparation protocol were highly concordant. The protocol described herein represents an effective and relatively sensitive means of preparing amplified nuclear DNA for massively parallel sequencing (MPS).

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalInternational journal of legal medicine
Volume129
Issue number1
DOIs
StatePublished - 1 Jan 2014

Keywords

  • Ancestry informative markers
  • Massively parallel sequencing
  • Phenotypic SNPs
  • TruSeq custom amplicon

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    Warshauer, D. H., Davis, C. P., Holt, C., Han, Y., Walichiewicz, P., Richardson, T., Stephens, K., Jager, A., King, J., & Budowle, B. (2014). Massively parallel sequencing of forensically relevant single nucleotide polymorphisms using TruSeq™ forensic amplicon. International journal of legal medicine, 129(1), 31-36. https://doi.org/10.1007/s00414-014-1108-8