Abstract
There is a body of evidence lending credence to the idea that oxidative stress may be responsible for age-related deleterious changes in brain function, and that protein carbonylation is a potential marker for such changes. An investigation of oxidative damage to mitochondrial proteins from aged rat brains was done using gel electrophoresis coupled with carbonylation-specific immunostaining. Six proteins that appeared to be susceptible to oxidative modification were identified by in-gel trypsin digestion followed by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry. Two subunits of the H+-transporting ATP synthase, adenine nucleotide translocator, voltage-dependent anion channel, glutamate oxaloacetate transaminase, and aconitase were identified as likely targets of age-associated carbonylation.
Original language | English |
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Pages (from-to) | 1583-1589 |
Number of pages | 7 |
Journal | Journal of Mass Spectrometry |
Volume | 42 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2007 |
Keywords
- Aging
- Gel electrophoresis
- Matrix-assisted laser desorption/ionization
- Mitochondria
- Oxidative stress
- Protein carbonylation
- Proteomics
- Tandem mass spectrometry