Maintenance of recombinant type a γ-aminobutyric acid receptor function

Role of protein tyrosine phosphorylation and calcineurin

Ren-Qi Huang, Glenn H. Dillon

Research output: Contribution to journalArticleResearchpeer-review

56 Citations (Scopus)

Abstract

In the present study, rundown of γ-aminobutyric acid (GABA)activated Cl- channels was studied in recombinant GABA(A) receptors stably expressed in human embryonic kidney cells (HEK 293), with conventional whole-cell and amphotericin B-perforated patch recording. When [ATP](i) was lowered to 1 mM and resting [Ca++](i) was buffered to a relatively high level, the response of α3 β2 γ2 GABA(A) receptors to relatively low [GABA] (up to 50 μM) did not show rundown in the whole-cell configuration. However, high [GABA] (greater than 200 μM) induced significant rundown, which was observed by decreases in both the maximum GABA-induced current and GABA EC50. Rundown was prevented completely with a solution containing 4 mM Mg++-ATP and low resting [Ca++](i), or during perforated patch recording. The magnitude of rundown was comparable in α1 β2 γ2 and β2 γ2 receptors. Neither stimulation nor inhibition of protein kinase A or protein kinase C had a significant effect on rundown. However, sodium metavanadate, an inhibitor of protein tyrosine phosphatase, significantly reduced rundown. In addition, inhibition of protein tyrosine kinase activity by either genistein or lavendustin A induced rundown of the GABA response. Inhibition of the Ca++/calmodulin-dependent phosphatase calcineurin with fenvalerate also prevented rundown of the response to GABA. Our results demonstrate that rundown of GABA(A) receptor function is concentration-dependent, due to depletion of ATP and/or unbuffered [Ca++](i), and does not depend on the presence or subtype of the alpha subunit. We propose that protein phosphorylation at a tyrosine kinase-dependent site, and a distinct unidentified site, which is dephosphorylated by calcineurin, maintains the function of GABA(A) receptors.

Original languageEnglish
Pages (from-to)243-255
Number of pages13
JournalJournal of Pharmacology and Experimental Therapeutics
Volume286
Issue number1
StatePublished - 1 Jul 1998

Fingerprint

Aminobutyrates
Calcineurin
gamma-Aminobutyric Acid
Tyrosine
Maintenance
Phosphorylation
GABA-A Receptors
Proteins
Adenosine Triphosphate
fenvalerate
Protein-Tyrosine Kinases
Protein Tyrosine Phosphatases
Vanadates
Genistein
HEK293 Cells
Amphotericin B
Calmodulin
Cyclic AMP-Dependent Protein Kinases
Protein Kinase C
Sodium

Cite this

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title = "Maintenance of recombinant type a γ-aminobutyric acid receptor function: Role of protein tyrosine phosphorylation and calcineurin",
abstract = "In the present study, rundown of γ-aminobutyric acid (GABA)activated Cl- channels was studied in recombinant GABA(A) receptors stably expressed in human embryonic kidney cells (HEK 293), with conventional whole-cell and amphotericin B-perforated patch recording. When [ATP](i) was lowered to 1 mM and resting [Ca++](i) was buffered to a relatively high level, the response of α3 β2 γ2 GABA(A) receptors to relatively low [GABA] (up to 50 μM) did not show rundown in the whole-cell configuration. However, high [GABA] (greater than 200 μM) induced significant rundown, which was observed by decreases in both the maximum GABA-induced current and GABA EC50. Rundown was prevented completely with a solution containing 4 mM Mg++-ATP and low resting [Ca++](i), or during perforated patch recording. The magnitude of rundown was comparable in α1 β2 γ2 and β2 γ2 receptors. Neither stimulation nor inhibition of protein kinase A or protein kinase C had a significant effect on rundown. However, sodium metavanadate, an inhibitor of protein tyrosine phosphatase, significantly reduced rundown. In addition, inhibition of protein tyrosine kinase activity by either genistein or lavendustin A induced rundown of the GABA response. Inhibition of the Ca++/calmodulin-dependent phosphatase calcineurin with fenvalerate also prevented rundown of the response to GABA. Our results demonstrate that rundown of GABA(A) receptor function is concentration-dependent, due to depletion of ATP and/or unbuffered [Ca++](i), and does not depend on the presence or subtype of the alpha subunit. We propose that protein phosphorylation at a tyrosine kinase-dependent site, and a distinct unidentified site, which is dephosphorylated by calcineurin, maintains the function of GABA(A) receptors.",
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Maintenance of recombinant type a γ-aminobutyric acid receptor function : Role of protein tyrosine phosphorylation and calcineurin. / Huang, Ren-Qi; Dillon, Glenn H.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 286, No. 1, 01.07.1998, p. 243-255.

Research output: Contribution to journalArticleResearchpeer-review

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