Maine caucasian population DNA database using twelve short tandem repeat loci

Timothy D. Kupferschmid, Theresa Calicchio, Bruce Budowle

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A population study of Caucasians residing in Maine was conducted using the AmpF1STR(TM) Profiler PCR Amplification Kit and the AmpF1STR(TM) Profiler Plus PCR Amplification Kit (Applied Biosystems Division (ABD) of Perkin Elmer, Foster City, CA). The kits contain the reagents necessary to amplify 12 different STR loci and the gender marker Amelogenin using two multiplex PCR, each containing nine STR loci. Thus, there is an overlap of six STR loci. The 12 STR loci are TH01, TPOX, CSF1PO, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, and D7S820. These loci represent 12 of the 13 core loci selected by the CODIS STR standardization project. Dye-labeled amplification products were separated and detected using the capillary electrophoresis instrument ABI Prism(TM) 310 Genetic Analyzer. Allele frequencies were determined for the 12 STR loci. Statistical analysis of the data included Hardy-Weinburg equilibrium (HWE) analysis, pairwise independence testing, power of discrimination (PD), and probability of exclusion (PE).

Original languageEnglish
Pages (from-to)392-395
Number of pages4
JournalJournal of Forensic Sciences
Volume44
Issue number2
DOIs
StatePublished - Mar 1999

Keywords

  • CSF1PO
  • D13S317
  • D18S51
  • D21S11
  • D3S1358
  • D5S818
  • D7 S820
  • D8S1179
  • DNA typing
  • FGA
  • Forensic science
  • Population database
  • Population genetics
  • Short tandem repeat loci
  • TH01
  • TPOX
  • vWA

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