TY - JOUR
T1 - Lymphatic pump treatment as an adjunct to antibiotics for pneumonia in a rat model
AU - Hodge, Lisa M.
AU - Creasy, Caitlin
AU - Carter, Kiah Rae
AU - Orlowski, Ashley
AU - Schander, Artur
AU - King, Hollis H.
N1 - Funding Information:
Support: Funding for this study was provided by the National Institutes of Health (grant AT004361) and the American Osteopathic Association (grants 13-11-687 and 10-11-609).
Publisher Copyright:
© 2015 American Osteopathic Association.
PY - 2015/5
Y1 - 2015/5
N2 - Background: Lymphatic pump treatment (LPT) is a technique used by osteopathic physicians as an adjunct to antibiotics for patients with respiratory tract infections, and previous studies have demonstrated that LPT reduces bacterial load in the lungs of rats with pneumonia. Currently, it is unknown whether LPT affects drug efficacy. Objective: To determine whether the combination of antibiotics and LPT would reduce bacterial load in the lungs of rats with acute pneumonia. Methods: Rats were infected intranasally with 5x107 colony-forming units (CFU) of Streptococcus pneumoniae. At 24, 48, and 72 hours after infection, the rats received no therapy (control), 4 minutes of sham therapy, or 4 minutes of LPT, followed by subcutaneous injection of 40 mg/kg of levofloxacin or sterile phosphate-buffered saline. At 48, 72, and 96 hours after infection, the spleens and lungs were collected, and S pneumoniae CFU were enumerated. Blood was analyzed for a complete blood cell count and leukocyte differential count. Results: At 48 and 72 hours after infection, no statistically significant differences in pulmonary CFU were found between control, sham therapy, or LPT when phosphate-buffered saline was administered; however, the reduction in CFU was statistically significant in all rats given levofloxacin. The combination of sham therapy and levofloxacin decreased bacterial load at 72 and 96 hours after infection, and LPT and levofloxacin significantly reduced CFU compared with sham therapy and levofloxacin at both time points (P<.05). Colony-forming units were not detected in the spleens at any time. No statistically significant differences in hematologic findings between any treatment groups were found at any time point measured. Conclusion: The results suggest that 3 applications of LPT induces an additional protective mechanism when combined with levofloxacin and support its use as an adjunctive therapy for the management of pneumonia; however, the mechanism responsible for this protection is unclear.
AB - Background: Lymphatic pump treatment (LPT) is a technique used by osteopathic physicians as an adjunct to antibiotics for patients with respiratory tract infections, and previous studies have demonstrated that LPT reduces bacterial load in the lungs of rats with pneumonia. Currently, it is unknown whether LPT affects drug efficacy. Objective: To determine whether the combination of antibiotics and LPT would reduce bacterial load in the lungs of rats with acute pneumonia. Methods: Rats were infected intranasally with 5x107 colony-forming units (CFU) of Streptococcus pneumoniae. At 24, 48, and 72 hours after infection, the rats received no therapy (control), 4 minutes of sham therapy, or 4 minutes of LPT, followed by subcutaneous injection of 40 mg/kg of levofloxacin or sterile phosphate-buffered saline. At 48, 72, and 96 hours after infection, the spleens and lungs were collected, and S pneumoniae CFU were enumerated. Blood was analyzed for a complete blood cell count and leukocyte differential count. Results: At 48 and 72 hours after infection, no statistically significant differences in pulmonary CFU were found between control, sham therapy, or LPT when phosphate-buffered saline was administered; however, the reduction in CFU was statistically significant in all rats given levofloxacin. The combination of sham therapy and levofloxacin decreased bacterial load at 72 and 96 hours after infection, and LPT and levofloxacin significantly reduced CFU compared with sham therapy and levofloxacin at both time points (P<.05). Colony-forming units were not detected in the spleens at any time. No statistically significant differences in hematologic findings between any treatment groups were found at any time point measured. Conclusion: The results suggest that 3 applications of LPT induces an additional protective mechanism when combined with levofloxacin and support its use as an adjunctive therapy for the management of pneumonia; however, the mechanism responsible for this protection is unclear.
UR - http://www.scopus.com/inward/record.url?scp=84961374926&partnerID=8YFLogxK
U2 - 10.7556/jaoa.2015.061
DO - 10.7556/jaoa.2015.061
M3 - Article
C2 - 25938525
AN - SCOPUS:84961374926
SN - 0098-6151
VL - 115
SP - 306
EP - 316
JO - Journal of the American Osteopathic Association
JF - Journal of the American Osteopathic Association
IS - 5
ER -