Lung, aorta, and platelet metabolism of 14C-arachidonic acid in vitamin E deficient rats

M. A. Valentovic, C. Gairola, W. C. Lubawy

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23 Scopus citations

Abstract

14C-arachidonic acid metabolism was determined in aortas, platelets, and perfused lungs from rats pair fed a basal diet supplemented with 0 or 100 ppm vitamin E for 11 weeks. Spontaneous erythrocyte hemolysis tests showed 92% and 8% hemolysis for the 0 and 100 ppm vitamin E groups, respectively. Elevated lung homogenate levels of malonaldehyde in the 0 ppm group confirmed its deficient group synthesized 54% less prostacyclin than aortas from the supplemented group (p<0.05). Although thromboxane generation by platelets from the vitamin E deficient group exhibited a 37% increase, this difference was not statistically significant compared to the supplemented animals Greater amounts of PGE2, PGF2, TXB2, and 6-keto-PGI were obtained in albumin buffer perfusates from lungs of vitamin E deficient rats than in those from supplemented rats. Significant differences (p<0.05) were noticed, however, only for PGE2 and PGF. These studies indicate that vitamin E quantitatively alters arachidonic acid metabolism in aortic and lung tissue but its effect on thrmboxane synthesis by platelets is less marked.

Original languageEnglish
Pages (from-to)215-224
Number of pages10
JournalProstaglandins
Volume24
Issue number2
DOIs
StatePublished - Aug 1982

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