Loop diuretics attenuate bradykinin-induced increase in clearance of macromolecules in the oral mucosa

Xiao Pei Gao, J. Michael Conlon, Jamboor K. Vishwanatha, Richard A. Robbins, Israel Rubinstein

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The purpose of this study was to determine whether loop diuretics attenuate bradykinin-induced increase in clearance of macromolecules in the oral mucosa in situ and, if so, to start to determine the mechanisms that mediated these responses. By using intravital microscopy, we found that bradykinin induced a significant concentration-dependent increase in fluorescein isothiocyanate-labeled dextran (mol mass 70 kDa) leaky site formation in the hamster cheek pouch. These responses were significantly attenuated by topical application of two structurally distinct loop diuretics, furosemide and ethacrynic acid, onto the cheek pouch (P < 0.05). Hydrochlorothiazide, a nonloop diuretic, had no significant effects on bradykinin-induced responses. Furosemide had no significant effects on adenosine-induced leaky site formation. Application of bradykinin after furosemide, but not after hydrochlorothiazide, was associated with a significant concentration-dependent decrease in bradykinin-like immunoreactivity in the cheek pouch suffusate (P < 0.05). Prostaglandins and changes in vasomotor tone did not modulate the effects of furosemide on bradykinin-induced responses. These data indicate that loop diuretics attenuate bradykinin-induced increase in clearance of macromolecules in the oral mucosa in a specific fashion, probably by amplifying local bradykinin catabolism. We suggest that topical loop diuretics could be useful in the treatment of oral mucosa inflammation elicited by bradykinin.

Original languageEnglish
Pages (from-to)818-823
Number of pages6
JournalJournal of Applied Physiology
Volume80
Issue number3
DOIs
StatePublished - Mar 1996

Keywords

  • ethacrynic acid
  • furosemide
  • hydrochlorothiazide
  • microcirculation
  • plasma exudation
  • radioimmunoassay

Fingerprint Dive into the research topics of 'Loop diuretics attenuate bradykinin-induced increase in clearance of macromolecules in the oral mucosa'. Together they form a unique fingerprint.

  • Cite this