TY - JOUR
T1 - LncRNAs expression in adjuvant-induced arthritis rats reveals the potential role of LncRNAs contributing to rheumatoid arthritis pathogenesis
AU - Jiang, Hui
AU - Qin, Xiu Juan
AU - Li, Wei Ping
AU - Ma, Rong
AU - Wang, Ting
AU - Li, Zhu Qing
N1 - Funding Information:
This study was financially supported by National Key Technology Support Program ( 2012BAI26B03 ). We are grateful to Mr. Hui Wang (Kang Chen Bio-tech Co., Ltd., Shanghai, China), and Mr. Qiang Fan (Ao Ji Bio-tech Co., Ltd., Shanghai, China) for providing helps in data analysis.
Publisher Copyright:
© 2016
PY - 2016/11/15
Y1 - 2016/11/15
N2 - Background Long non-coding RNAs (LncRNAs) are an important class of widespread molecules involved in diverse biological functions, which are exceptionally expressed in numerous types of diseases. Currently, limited study on LncRNA in rheumatoid arthritis (RA) is available. In this study, we aimed to identify the specifically expressed LncRNA that are relevant to adjuvant-induced arthritis (AA) in rats, and to explore the possible molecular mechanisms of RA pathogenesis. Methods To identify LncRNAs specifically expressed in rheumatoid arthritis, the expression of LncRNAs in synoviums of rats from the model group (n = 3) was compared with that in the control group (n = 3) using Arraystar Rat LncRNA/mRNA microarray and real-time polymerase chain reaction (RT-PCR). Results Up to 260 LncRNAs were found to be differentially expressed (≥ 1.5-fold-change) in the synoviums between AA model and the normal rats (170 up-regulated and 90 down-regulated LncRNAs in AA rats compared with normal rats). Coding-non-coding gene co-expression networks (CNC network) were drawn based on the correlation analysis between the differentially expressed LncRNAs and mRNAs. Six LncRNAs, XR_008357, U75927, MRAK046251, XR_006457, DQ266363 and MRAK003448, were selected to analyze the relationship between LncRNAs and RA via the CNC network and GO analysis. Real-time PCR result confirmed that the six LncRNAs were specifically expressed in the AA rats. Conclusions These results revealed that clusters of LncRNAs were uniquely expressed in AA rats compared with controls, which manifests that these differentially expressed LncRNAs in AA rats might play a vital role in RA development. Up-regulation or down-regulation of the six LncRNAs might contribute to the molecular mechanism underlying RA. To sum up, our study provides potential targets for treatment of RA and novel profound understanding of the pathogenesis of RA.
AB - Background Long non-coding RNAs (LncRNAs) are an important class of widespread molecules involved in diverse biological functions, which are exceptionally expressed in numerous types of diseases. Currently, limited study on LncRNA in rheumatoid arthritis (RA) is available. In this study, we aimed to identify the specifically expressed LncRNA that are relevant to adjuvant-induced arthritis (AA) in rats, and to explore the possible molecular mechanisms of RA pathogenesis. Methods To identify LncRNAs specifically expressed in rheumatoid arthritis, the expression of LncRNAs in synoviums of rats from the model group (n = 3) was compared with that in the control group (n = 3) using Arraystar Rat LncRNA/mRNA microarray and real-time polymerase chain reaction (RT-PCR). Results Up to 260 LncRNAs were found to be differentially expressed (≥ 1.5-fold-change) in the synoviums between AA model and the normal rats (170 up-regulated and 90 down-regulated LncRNAs in AA rats compared with normal rats). Coding-non-coding gene co-expression networks (CNC network) were drawn based on the correlation analysis between the differentially expressed LncRNAs and mRNAs. Six LncRNAs, XR_008357, U75927, MRAK046251, XR_006457, DQ266363 and MRAK003448, were selected to analyze the relationship between LncRNAs and RA via the CNC network and GO analysis. Real-time PCR result confirmed that the six LncRNAs were specifically expressed in the AA rats. Conclusions These results revealed that clusters of LncRNAs were uniquely expressed in AA rats compared with controls, which manifests that these differentially expressed LncRNAs in AA rats might play a vital role in RA development. Up-regulation or down-regulation of the six LncRNAs might contribute to the molecular mechanism underlying RA. To sum up, our study provides potential targets for treatment of RA and novel profound understanding of the pathogenesis of RA.
KW - LncRNAs
KW - Microarray
KW - Molecular mechanism
KW - Rheumatoid arthritis
KW - mRNA
UR - http://www.scopus.com/inward/record.url?scp=84990198798&partnerID=8YFLogxK
U2 - 10.1016/j.gene.2016.08.012
DO - 10.1016/j.gene.2016.08.012
M3 - Article
C2 - 27511374
AN - SCOPUS:84990198798
SN - 0378-1119
VL - 593
SP - 131
EP - 142
JO - Gene
JF - Gene
IS - 1
ER -