Light-induced isomerization of the LHCII-bound xanthophyll neoxanthin: Possible implications for photoprotection in plants

Monika Zubik, Rafał Luchowski, Wojciech Grudzinski, Małgorzata Gospodarek, Ignacy Gryczynski, Zygmunt Gryczynski, Jerzy W. Dobrucki, Wiesław I. Gruszecki

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Light-harvesting pigment-protein complex of Photosystem II (LHCII) is the largest photosynthetic antenna complex of plants and the most abundant membrane protein in the biosphere. Plant fitness and productivity depend directly on a balance between excitations in the photosynthetic apparatus, generated by captured light quanta, and the rate of photochemical processes. Excess excitation energy leads to oxidative damage of the photosynthetic apparatus and entire organism and therefore the balance between the excitation density and photosynthesis requires precise and efficient regulation, operating also at the level of antenna complexes. We show that illumination of the isolated LHCII leads to isomerization of the protein-bound neoxanthin from conformation 9′-cis to 9′,13- and 9′,13′-dicis forms. At the same time light-driven excitation quenching is observed, manifested by a decrease in chlorophyll a fluorescence intensity and shortened fluorescence lifetimes. Both processes, the neoxanthin isomerization and the chlorophyll excitation quenching, are reversible in dim light. The results of the 77 K florescence measurements of LHCII show that illumination is associated with appearance of the low-energy states, which can serve as energy traps in the pigment-protein complex subjected to excess excitation. Possible sequence of the molecular events is proposed, leading to a protective excess excitation energy quenching: neoxanthin photo-isomerization → formation of LHCII supramolecular structures which potentiate creation of energy traps → excitation quenching.

Original languageEnglish
Pages (from-to)1237-1243
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1807
Issue number9
DOIs
StatePublished - 1 Sep 2011

Fingerprint

Xanthophylls
Isomerization
Quenching
Lighting
Light
Excitation energy
Light-Harvesting Protein Complexes
Photochemical Processes
Fluorescence
Pigments
Photosynthetic Reaction Center Complex Proteins
Photosystem II Protein Complex
Photosynthesis
Chlorophyll
Antennas
Photoisomerization
Membrane Proteins
Proteins
Electron energy levels
Conformations

Keywords

  • Fluorescence
  • LHCII
  • Photoprotection
  • Photosynthesis
  • Spectroscopy
  • Xanthophyll

Cite this

Zubik, Monika ; Luchowski, Rafał ; Grudzinski, Wojciech ; Gospodarek, Małgorzata ; Gryczynski, Ignacy ; Gryczynski, Zygmunt ; Dobrucki, Jerzy W. ; Gruszecki, Wiesław I. / Light-induced isomerization of the LHCII-bound xanthophyll neoxanthin : Possible implications for photoprotection in plants. In: Biochimica et Biophysica Acta - Bioenergetics. 2011 ; Vol. 1807, No. 9. pp. 1237-1243.
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Light-induced isomerization of the LHCII-bound xanthophyll neoxanthin : Possible implications for photoprotection in plants. / Zubik, Monika; Luchowski, Rafał; Grudzinski, Wojciech; Gospodarek, Małgorzata; Gryczynski, Ignacy; Gryczynski, Zygmunt; Dobrucki, Jerzy W.; Gruszecki, Wiesław I.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1807, No. 9, 01.09.2011, p. 1237-1243.

Research output: Contribution to journalArticle

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T1 - Light-induced isomerization of the LHCII-bound xanthophyll neoxanthin

T2 - Possible implications for photoprotection in plants

AU - Zubik, Monika

AU - Luchowski, Rafał

AU - Grudzinski, Wojciech

AU - Gospodarek, Małgorzata

AU - Gryczynski, Ignacy

AU - Gryczynski, Zygmunt

AU - Dobrucki, Jerzy W.

AU - Gruszecki, Wiesław I.

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KW - LHCII

KW - Photoprotection

KW - Photosynthesis

KW - Spectroscopy

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