Kinetic and spectral studies of type I and type II compounds with rat hepatic microsomes in the presence of the major metabolite of diphenylhydantoin

S. A. Stavchansky, H. B. Kostenbauder, W. C. Lubawy

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6 Scopus citations

Abstract

The nature of the inhibitory effects of the major metabolite of diphenylhydantoin, 5 (p hydroxyphenyl) 5 phenylhydantoin (HPPH), on the in vitro metabolism of ethylmorphine and aniline by rat hepatic microsomes was examined. The N demethylation of ethylmorphine was competitively inhibited by HPPH, whereas inhibition of the hydroxylation of aniline was not competitive. The spectrum produced by HPPH when added to microsomal suspensions does not resemble the classical type I or type II spectra, but rather a reversed type I spectrum. Spectral evidence is presented indicating that HPPH also diminishes the magnitude of the spectral change produced by type I and II compounds.

Original languageEnglish
Pages (from-to)557-564
Number of pages8
JournalDrug Metabolism and Disposition
Volume3
Issue number6
StatePublished - 1 Dec 1975

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