Italian population data on the polymarker system and on the five short tandem repeat loci CSF1PO, TPOX, TH01, F13B, and vWA

Luciano Garofano; Gianpietro Lago; Cesare Vecchio; Marco Pizzamiglio; Carlo Zanon; Antonino Virgili; Loredana Albonici; Vittorio Manzari; Bruce Budowle

doi:10.1520/jfs14315j

Italian population data on the polymarker system and on the five short tandem repeat loci CSF1PO, TPOX, TH01, F13B, and vWA

Luciano Garofano, Gianpietro Lago, Cesare Vecchio, Marco Pizzamiglio, Carlo Zanon, Antonino Virgili, Loredana Albonici, Vittorio Manzari, Bruce Budowle

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A population study on five short tandem repeat (STR) loci and five sequence specific polymorphism loci was performed on unrelated Italian Caucasians. Separation and detection of the amplified STR fragments were carried out by high resolution vertical denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining, respectively. The sequence specific loci were analyzed using the AmpliType PM Typing Kit (Perkin Elmer, Foster City, CA). All loci, except Gc (p = 0.031), meet Hardy-Wienberg expectations. In addition, there is no evidence for association of alleles between pairs of loci. The combined power of discrimination for the five STR loci is 0.9999862 and for the PM loci is 0.99503. The results suggest that these loci may be useful for human identification cases in Italy.

Original language	English
Pages (from-to)	837-840
Number of pages	4
Journal	Journal of Forensic Sciences
Volume	43
Issue number	4
DOIs	https://doi.org/10.1520/jfs14315j
State	Published - Jul 1998

Keywords

D7S8
DNA typing
Forensic science
GYPA
Gc
HBGG
HumCSF1PO
HumF13B
HumTH01
HumTPOX
HumvWA31
Ital y
LDLR
Population genetics

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@article{f64fe41be53c47918662a5abd7441f00,

title = "Italian population data on the polymarker system and on the five short tandem repeat loci CSF1PO, TPOX, TH01, F13B, and vWA",

abstract = "A population study on five short tandem repeat (STR) loci and five sequence specific polymorphism loci was performed on unrelated Italian Caucasians. Separation and detection of the amplified STR fragments were carried out by high resolution vertical denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining, respectively. The sequence specific loci were analyzed using the AmpliType PM Typing Kit (Perkin Elmer, Foster City, CA). All loci, except Gc (p = 0.031), meet Hardy-Wienberg expectations. In addition, there is no evidence for association of alleles between pairs of loci. The combined power of discrimination for the five STR loci is 0.9999862 and for the PM loci is 0.99503. The results suggest that these loci may be useful for human identification cases in Italy.",

keywords = "D7S8, DNA typing, Forensic science, GYPA, Gc, HBGG, HumCSF1PO, HumF13B, HumTH01, HumTPOX, HumvWA31, Ital y, LDLR, Population genetics",

author = "Luciano Garofano and Gianpietro Lago and Cesare Vecchio and Marco Pizzamiglio and Carlo Zanon and Antonino Virgili and Loredana Albonici and Vittorio Manzari and Bruce Budowle",

year = "1998",

month = jul,

doi = "10.1520/jfs14315j",

language = "English",

volume = "43",

pages = "837--840",

journal = "Journal of forensic sciences",

issn = "0022-1198",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Italian population data on the polymarker system and on the five short tandem repeat loci CSF1PO, TPOX, TH01, F13B, and vWA

AU - Garofano, Luciano

AU - Lago, Gianpietro

AU - Vecchio, Cesare

AU - Pizzamiglio, Marco

AU - Zanon, Carlo

AU - Virgili, Antonino

AU - Albonici, Loredana

AU - Manzari, Vittorio

AU - Budowle, Bruce

PY - 1998/7

Y1 - 1998/7

N2 - A population study on five short tandem repeat (STR) loci and five sequence specific polymorphism loci was performed on unrelated Italian Caucasians. Separation and detection of the amplified STR fragments were carried out by high resolution vertical denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining, respectively. The sequence specific loci were analyzed using the AmpliType PM Typing Kit (Perkin Elmer, Foster City, CA). All loci, except Gc (p = 0.031), meet Hardy-Wienberg expectations. In addition, there is no evidence for association of alleles between pairs of loci. The combined power of discrimination for the five STR loci is 0.9999862 and for the PM loci is 0.99503. The results suggest that these loci may be useful for human identification cases in Italy.

AB - A population study on five short tandem repeat (STR) loci and five sequence specific polymorphism loci was performed on unrelated Italian Caucasians. Separation and detection of the amplified STR fragments were carried out by high resolution vertical denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining, respectively. The sequence specific loci were analyzed using the AmpliType PM Typing Kit (Perkin Elmer, Foster City, CA). All loci, except Gc (p = 0.031), meet Hardy-Wienberg expectations. In addition, there is no evidence for association of alleles between pairs of loci. The combined power of discrimination for the five STR loci is 0.9999862 and for the PM loci is 0.99503. The results suggest that these loci may be useful for human identification cases in Italy.

KW - D7S8

KW - DNA typing

KW - Forensic science

KW - GYPA

KW - Gc

KW - HBGG

KW - HumCSF1PO

KW - HumF13B

KW - HumTH01

KW - HumTPOX

KW - HumvWA31

KW - Ital y

KW - LDLR

KW - Population genetics

UR - http://www.scopus.com/inward/record.url?scp=0031926161&partnerID=8YFLogxK

U2 - 10.1520/jfs14315j

DO - 10.1520/jfs14315j

M3 - Article

C2 - 9670508

AN - SCOPUS:0031926161

SN - 0022-1198

VL - 43

SP - 837

EP - 840

JO - Journal of forensic sciences

JF - Journal of forensic sciences

IS - 4

ER -

Italian population data on the polymarker system and on the five short tandem repeat loci CSF1PO, TPOX, TH01, F13B, and vWA

Abstract

Keywords

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