Isolation of high-density lipoproteins by immunoaffinity column chromatography from hog plasma

Yong B. Park, Mehrnoosh Jahani, Andras G. Lacko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. 2. 2. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. 3. 3. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components than the control as indicated by acrylamide gel electrophoresis in the presence of urea. 4. 4. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.

Original languageEnglish
Pages (from-to)529-533
Number of pages5
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume82
Issue number3
DOIs
StatePublished - 1 Jan 1985

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Column chromatography
HDL Lipoproteins
Chromatography
Plasmas
Ultracentrifugation
Gels
Acrylamide
Apolipoprotein A-I
Apolipoproteins E
Electrophoresis
Gel Chromatography
Urea
Peptides
Chemical analysis
Proteins

Cite this

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abstract = "1. 1. High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. 2. 2. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. 3. 3. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components than the control as indicated by acrylamide gel electrophoresis in the presence of urea. 4. 4. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.",
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Isolation of high-density lipoproteins by immunoaffinity column chromatography from hog plasma. / Park, Yong B.; Jahani, Mehrnoosh; Lacko, Andras G.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 82, No. 3, 01.01.1985, p. 529-533.

Research output: Contribution to journalArticle

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AB - 1. 1. High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. 2. 2. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. 3. 3. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components than the control as indicated by acrylamide gel electrophoresis in the presence of urea. 4. 4. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.

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