Isolation of high-density lipoproteins by immunoaffinity column chromatography from hog plasma

1. 1. High density lipoprotein (HDL) was isolated from hog plasma by a simple immunoaffinity column chromatography procedure using immobilized anti-apolipoprotein AI. 2. 2. The composition of HDL isolated by immunoaffinity chromatography was nearly identical to that of a control sample that was isolated by an alternate method utilizing ultracentrifugation and gel chromatography. 3. 3. The HDL isolated by immunoaffinity chromatography had a larger number of polypeptide components than the control as indicated by acrylamide gel electrophoresis in the presence of urea. 4. 4. When the HDL isolated by immunoaffinity chromatography was applied to a heparin-agarose column the amount of protein retained was approximately twice that of the control. These findings indicate that the ultracentrifugation procedure probably induced the loss of apolipoprotein E containing components from the HDL complex.