Isolation and Culture of Rhesus Adipose-Derived Stem Cells

Christine Gagliardi, Bruce A. Bunnell

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

12 Scopus citations


Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Rhesus monkeys are physiologically and phylogenetically similar to humans, and they and their cells are valuable for biomedical research and evaluation of preclinical therapies. Here, we describe methods for the isolation, culture, and differentiation of rhesus adipose-derived stem cells (rASCs).

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Adipose-derived stem cells (ASCs)
  • Collagenase
  • Expansion
  • Isolation
  • Mesenchymal stem cells (MSCs)
  • Rhesus macaque
  • Stromal vascular fraction (SVF)


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