Ischemia-induced angiogenesis is attenuated in aged rats

Yaohui Tang; Liuqing Wang; Jixian Wang; Xiaojie Lin; Yongting Wang; Kunlin Jin; Guo Yuan Yang

doi:10.14336/AD.2015.1125

Ischemia-induced angiogenesis is attenuated in aged rats

Yaohui Tang, Liuqing Wang, Jixian Wang, Xiaojie Lin, Yongting Wang, Kunlin Jin, Guo Yuan Yang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain. In addition, we found that after MCAO, the number of blood vessels in the peri-infarct region of ipsilateral hemisphere in aged ischemic rats was significantly increased compared to the aged sham rats (p<0.05). We also confirmed that ischemia-induced focal angiogenesis occurred in young adult rat brain while the blood vessel density in young adult ischemic brain was significantly higher than that in the aged ischemic brain (p<0.05). Our data suggests that focal angiogenesis in aged rat brain can be induced in response to ischemic brain injury, and that aging impedes brain repairing and remodeling after ischemic stroke, possible due to the limited response of angiogenesis.

Original language	English
Pages (from-to)	326-335
Number of pages	10
Journal	Aging and Disease
Volume	7
Issue number	4
DOIs	https://doi.org/10.14336/AD.2015.1125
State	Published - 2016

Keywords

Aging angiogenesis
Brain
Focal cerebral ischemia
Rat

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10.14336/AD.2015.1125

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title = "Ischemia-induced angiogenesis is attenuated in aged rats",

abstract = "To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain. In addition, we found that after MCAO, the number of blood vessels in the peri-infarct region of ipsilateral hemisphere in aged ischemic rats was significantly increased compared to the aged sham rats (p<0.05). We also confirmed that ischemia-induced focal angiogenesis occurred in young adult rat brain while the blood vessel density in young adult ischemic brain was significantly higher than that in the aged ischemic brain (p<0.05). Our data suggests that focal angiogenesis in aged rat brain can be induced in response to ischemic brain injury, and that aging impedes brain repairing and remodeling after ischemic stroke, possible due to the limited response of angiogenesis.",

keywords = "Aging angiogenesis, Brain, Focal cerebral ischemia, Rat",

author = "Yaohui Tang and Liuqing Wang and Jixian Wang and Xiaojie Lin and Yongting Wang and Kunlin Jin and Yang, {Guo Yuan}",

note = "Funding Information: This study was supported by research grants from the National Natural Science Foundation of China (81070939, 81471178 and 81371305), National Basic Research Program of China (973 Program 2011CB504405), the Science and Technology Commission of Shanghai Municipality (10JC1408100) and KC Wong Foundation (GYY). We thank Minjie Shen for editorial assistance, and the staff of Neuroscience and Neuroengineering Center (http://med-x.sjtu.edu.cn/english), Shanghai Jiao Tong University for their collaborative support. Publisher Copyright: {\textcopyright} 2016 Tang Y, et al.",

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AU - Tang, Yaohui

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AU - Wang, Jixian

AU - Lin, Xiaojie

AU - Wang, Yongting

AU - Jin, Kunlin

AU - Yang, Guo Yuan

N1 - Funding Information: This study was supported by research grants from the National Natural Science Foundation of China (81070939, 81471178 and 81371305), National Basic Research Program of China (973 Program 2011CB504405), the Science and Technology Commission of Shanghai Municipality (10JC1408100) and KC Wong Foundation (GYY). We thank Minjie Shen for editorial assistance, and the staff of Neuroscience and Neuroengineering Center (http://med-x.sjtu.edu.cn/english), Shanghai Jiao Tong University for their collaborative support. Publisher Copyright: © 2016 Tang Y, et al.

PY - 2016

Y1 - 2016

N2 - To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain. In addition, we found that after MCAO, the number of blood vessels in the peri-infarct region of ipsilateral hemisphere in aged ischemic rats was significantly increased compared to the aged sham rats (p<0.05). We also confirmed that ischemia-induced focal angiogenesis occurred in young adult rat brain while the blood vessel density in young adult ischemic brain was significantly higher than that in the aged ischemic brain (p<0.05). Our data suggests that focal angiogenesis in aged rat brain can be induced in response to ischemic brain injury, and that aging impedes brain repairing and remodeling after ischemic stroke, possible due to the limited response of angiogenesis.

AB - To study whether focal angiogenesis is induced in aged rodents after permanent distal middle cerebral artery occlusion (MCAO), young adult (3-month-old) and aged (24-month-old) Fisher 344 rats underwent MCAO and sacrificed up to two months after MCAO. Immunohistochemistry and synchrotron radiation microangiography were performed to examine the number of newly formed blood vessels in both young adult and aged rats post-ischemia. We found that the number of capillaries and small arteries in aged brain was the same as young adult brain. In addition, we found that after MCAO, the number of blood vessels in the peri-infarct region of ipsilateral hemisphere in aged ischemic rats was significantly increased compared to the aged sham rats (p<0.05). We also confirmed that ischemia-induced focal angiogenesis occurred in young adult rat brain while the blood vessel density in young adult ischemic brain was significantly higher than that in the aged ischemic brain (p<0.05). Our data suggests that focal angiogenesis in aged rat brain can be induced in response to ischemic brain injury, and that aging impedes brain repairing and remodeling after ischemic stroke, possible due to the limited response of angiogenesis.

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Ischemia-induced angiogenesis is attenuated in aged rats

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