TY - JOUR
T1 - Is protein kinase C alpha (PKCα) involved in vasopressin-induced effects on LLC-PK1 pig kidney cells?
AU - Dibas, Adnan
AU - Mia, Abdul J.
AU - Yorio, Thomas
PY - 1996
Y1 - 1996
N2 - The involvement of protein kinase C (PKC) in vasopressin-induced effects on renal water reabsorption is still unresolved. Activation of PKC can be detected by its translocation from the cytosol (C) to the plasma membrane (PM). In LLC-PK1 cells, the redistribution of PKCα, a predominant isoform of PKC detected, was studied utilizing western blotting after stimulation with vasopressin. Vasopressin (100 mU/ml) failed to induce a translocation of PKCα from the C to the PM. By contrast, phorbol myristate acetate (PMA, 200 nM), a potent activator of PKC, induced a relocalization of PKCα from the C to the PM. After 2 hours of treatment of cells with PMA, PKCα was predominantly detected in the PM and absent from the C. These results suggest that the signal transduction pathway of vasopressin in LLC-PK1 cells does not involve PKCα activation and translocation.
AB - The involvement of protein kinase C (PKC) in vasopressin-induced effects on renal water reabsorption is still unresolved. Activation of PKC can be detected by its translocation from the cytosol (C) to the plasma membrane (PM). In LLC-PK1 cells, the redistribution of PKCα, a predominant isoform of PKC detected, was studied utilizing western blotting after stimulation with vasopressin. Vasopressin (100 mU/ml) failed to induce a translocation of PKCα from the C to the PM. By contrast, phorbol myristate acetate (PMA, 200 nM), a potent activator of PKC, induced a relocalization of PKCα from the C to the PM. After 2 hours of treatment of cells with PMA, PKCα was predominantly detected in the PM and absent from the C. These results suggest that the signal transduction pathway of vasopressin in LLC-PK1 cells does not involve PKCα activation and translocation.
KW - Kidney
KW - PKCα
KW - Translocation and phorbol ester
KW - Vasopressin
UR - http://www.scopus.com/inward/record.url?scp=0030055433&partnerID=8YFLogxK
U2 - 10.1080/15216549600201641
DO - 10.1080/15216549600201641
M3 - Article
C2 - 8828810
AN - SCOPUS:0030055433
SN - 1039-9712
VL - 39
SP - 581
EP - 588
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
IS - 3
ER -