Involvement of annexin II in DNA replication: Evidence from cell-free extracts of Xenopus eggs

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Abstract

Cell-free extracts of Xenopus eggs efficiently initiate and complete semiconservative DNA replication of exogenously added plasmid DNA. DNA replication in such extracts can be neutralized by a monoclonal antibody (D1/274.5) against human annexin II. Specific immunodepletion of Xenopus annexin II from the egg extracts results in loss of DNA replicative ability. Immunodepletion of annexin II does not prevent nuclear assembly, a stringent requirement for DNA synthesis on exogenous DNA in this system. Replicative ability can be restored to the immunodepleted extracts by the addition of purified human annexin II. These results demonstrate that annexin II is involved in chromosomal DNA replication and has a role in the cell cycle of higher eukaryotes.

Original languageEnglish
Pages (from-to)533-540
Number of pages8
JournalJournal of Cell Science
Volume105
Issue number2
StatePublished - 1 Jan 1993

Keywords

  • Annexin II
  • DNA replication
  • Primer recognition proteins

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