Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII

Wieslaw I. Gruszecki, Monika Zubik, Rafal Luchowski, Wojciech Grudzinski, Malgorzata Gospodarek, Janusz Szurkowski, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Excitation of the major photosynthetic antenna complex of plants, LHCII, with blue light (470. nm) provides an advantage to plants, as it gives rise to chlorophyll a fluorescence lifetimes shorter than with excitation with red light (635. nm). This difference is particularly pronounced in fluorescence emission wavelengths longer than 715. nm. Illumination of LHCII preparation with blue light additionally induces fluorescence quenching, which develops on a minute timescale. This effect is much less efficient when induced by red light, despite the equalized energy absorbed in both the spectral regions. Simultaneous analysis of the fluorescence and photoacoustic signals in LHCII demonstrated that the light-driven fluorescence quenching is not associated with an increase in heat emission. Instead, a reversible light-induced conformational transformation of the protein takes place, as demonstrated by the FTIR technique. These findings are discussed in terms of the blue-light-specific excitation energy quenching in LHCII, which may have photoprotective applications.

Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalJournal of Plant Physiology
Volume168
Issue number5
DOIs
StatePublished - 15 Mar 2011

Fingerprint

blue light
antennae
fluorescence
Light
energy
Fluorescence
red light
heat emissions
protein intake
Photosynthetic Reaction Center Complex Proteins
lighting
wavelengths
Fourier Transform Infrared Spectroscopy
Lighting
chlorophyll
Hot Temperature
Proteins

Keywords

  • Blue light effect
  • FLIM
  • LHCII
  • Photoprotection
  • Xanthophylls

Cite this

Gruszecki, Wieslaw I. ; Zubik, Monika ; Luchowski, Rafal ; Grudzinski, Wojciech ; Gospodarek, Malgorzata ; Szurkowski, Janusz ; Gryczynski, Zygmunt ; Gryczynski, Ignacy. / Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII. In: Journal of Plant Physiology. 2011 ; Vol. 168, No. 5. pp. 409-414.
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abstract = "Excitation of the major photosynthetic antenna complex of plants, LHCII, with blue light (470. nm) provides an advantage to plants, as it gives rise to chlorophyll a fluorescence lifetimes shorter than with excitation with red light (635. nm). This difference is particularly pronounced in fluorescence emission wavelengths longer than 715. nm. Illumination of LHCII preparation with blue light additionally induces fluorescence quenching, which develops on a minute timescale. This effect is much less efficient when induced by red light, despite the equalized energy absorbed in both the spectral regions. Simultaneous analysis of the fluorescence and photoacoustic signals in LHCII demonstrated that the light-driven fluorescence quenching is not associated with an increase in heat emission. Instead, a reversible light-induced conformational transformation of the protein takes place, as demonstrated by the FTIR technique. These findings are discussed in terms of the blue-light-specific excitation energy quenching in LHCII, which may have photoprotective applications.",
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Investigation of the molecular mechanism of the blue-light-specific excitation energy quenching in the plant antenna complex LHCII. / Gruszecki, Wieslaw I.; Zubik, Monika; Luchowski, Rafal; Grudzinski, Wojciech; Gospodarek, Malgorzata; Szurkowski, Janusz; Gryczynski, Zygmunt; Gryczynski, Ignacy.

In: Journal of Plant Physiology, Vol. 168, No. 5, 15.03.2011, p. 409-414.

Research output: Contribution to journalArticle

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AU - Gruszecki, Wieslaw I.

AU - Zubik, Monika

AU - Luchowski, Rafal

AU - Grudzinski, Wojciech

AU - Gospodarek, Malgorzata

AU - Szurkowski, Janusz

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

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