Intrinsic fluorescence from DNA can be enhanced by metallic particles

J. R. Lakowicz; B. Shen; Z. Gryczynski; S. D'Auria; I. Gryczynski

doi:10.1006/bbrc.2001.5445

Intrinsic fluorescence from DNA can be enhanced by metallic particles

J. R. Lakowicz, B. Shen, Z. Gryczynski, S. D'Auria, I. Gryczynski

Microbiology, Immunology & Genetics

Research output: Contribution to journal › Article › peer-review

193 Scopus citations

Abstract

High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Silver islands are subwavelength size patches of metallic silver on an inert substrate. Time-resolved measurements show a decreased lifetime for the intrinsic DNA emission near the silver islands. These results of increased intensity and decreased lifetime indicate a metal-induced increase in the radiative rate decay of the DNA bases. The possibility of increased radiative decay rates for DNA bases and other fluorophores suggest a wide variety of DNA measurements and other biomedical assays based on metal-induced increases in the fluorescence quantum yield of weakly fluorescent substances.

Original language	English
Pages (from-to)	875-879
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	286
Issue number	5
DOIs	https://doi.org/10.1006/bbrc.2001.5445
State	Published - 2001

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title = "Intrinsic fluorescence from DNA can be enhanced by metallic particles",

abstract = "High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Silver islands are subwavelength size patches of metallic silver on an inert substrate. Time-resolved measurements show a decreased lifetime for the intrinsic DNA emission near the silver islands. These results of increased intensity and decreased lifetime indicate a metal-induced increase in the radiative rate decay of the DNA bases. The possibility of increased radiative decay rates for DNA bases and other fluorophores suggest a wide variety of DNA measurements and other biomedical assays based on metal-induced increases in the fluorescence quantum yield of weakly fluorescent substances.",

author = "Lakowicz, {J. R.} and B. Shen and Z. Gryczynski and S. D'Auria and I. Gryczynski",

note = "Funding Information: This work was supported by a grant from the NIH, National Center for Research Resource, RR-08119, with partial support from the Juvenile Diabetes Foundation International and the American Diabetes Association.",

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doi = "10.1006/bbrc.2001.5445",

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T1 - Intrinsic fluorescence from DNA can be enhanced by metallic particles

AU - Lakowicz, J. R.

AU - Shen, B.

AU - Gryczynski, Z.

AU - D'Auria, S.

AU - Gryczynski, I.

N1 - Funding Information: This work was supported by a grant from the NIH, National Center for Research Resource, RR-08119, with partial support from the Juvenile Diabetes Foundation International and the American Diabetes Association.

PY - 2001

Y1 - 2001

N2 - High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Silver islands are subwavelength size patches of metallic silver on an inert substrate. Time-resolved measurements show a decreased lifetime for the intrinsic DNA emission near the silver islands. These results of increased intensity and decreased lifetime indicate a metal-induced increase in the radiative rate decay of the DNA bases. The possibility of increased radiative decay rates for DNA bases and other fluorophores suggest a wide variety of DNA measurements and other biomedical assays based on metal-induced increases in the fluorescence quantum yield of weakly fluorescent substances.

AB - High sensitivity detection of DNA is essential for genomics. The intrinsic fluorescence from DNA is very weak and almost all methods for detecting DNA rely on the use of extrinsic fluorescent probes. We show that the intrinsic emission from DNA can be enhanced many-fold by spatial proximity to silver island films. Silver islands are subwavelength size patches of metallic silver on an inert substrate. Time-resolved measurements show a decreased lifetime for the intrinsic DNA emission near the silver islands. These results of increased intensity and decreased lifetime indicate a metal-induced increase in the radiative rate decay of the DNA bases. The possibility of increased radiative decay rates for DNA bases and other fluorophores suggest a wide variety of DNA measurements and other biomedical assays based on metal-induced increases in the fluorescence quantum yield of weakly fluorescent substances.

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Intrinsic fluorescence from DNA can be enhanced by metallic particles

Abstract

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