TY - JOUR
T1 - Intrapericardial procaine affects volume expansion-induced Fos immunoreactivity in unanesthetized rats
AU - Cunningham, J. Thomas
AU - Bruno, Stacy B.
AU - Higgs, Karen A.N.
AU - Sullivan., Margaret J.
N1 - Funding Information:
This work was supported by National Heart, Lung and Blood Institute Grants R01-HL62579 (J.T.C.) and K02-HL03620 (J.T.C.), an NIDDK Grant, R01-DK57822 (M.J.S.), and a Grant-in-Aid from the Missouri Affiliate of the American Heart Association (J.T.C.). M.J.S. died June 2, 2001.
PY - 2002
Y1 - 2002
N2 - Acute volume expansion is associated with a specific pattern of Fos expression and the goal of the present study was to evaluate the contribution of cardiac receptors to this response. Adult male rats were instrumented with pericardial catheters introduced at the level of the thymus. Rats were also catheterized for measuring blood pressure, heart rate, central venous pressure, and intravenous infusion. Each rat received a 200-μl intrapericardial (i.p.c) injection of 2% procaine or 0.9% NaCl. Rats were then volume expanded with isotonic saline (10% body weight in 10 min) or given a control infusion (0.01 ml/min for 10 min). Ninety minutes after the start of the infusion, the rats were anesthetized and perfused transcardially. Their brains were sectioned and processed for Fos, dopamine-β-hydroxylase, and oxytocin immunocytochemistry. Volume expansion plus i.p.c. saline produced a significant increase in Fos expression in the nucleus of the solitary tract, the ventrolateral medulla, the area postrema, the locus coeruleus, the paraventricular nucleus of the hypothalamus, the perinuclear zone of the supraoptic nucleus, and oxytocin neurons in the supraoptic nucleus. The i.p.c. procaine significantly blocked Fos expression produced by the volume expansion in the all of the regions examined except for the area postrema and the SON oxytocin neurons.
AB - Acute volume expansion is associated with a specific pattern of Fos expression and the goal of the present study was to evaluate the contribution of cardiac receptors to this response. Adult male rats were instrumented with pericardial catheters introduced at the level of the thymus. Rats were also catheterized for measuring blood pressure, heart rate, central venous pressure, and intravenous infusion. Each rat received a 200-μl intrapericardial (i.p.c) injection of 2% procaine or 0.9% NaCl. Rats were then volume expanded with isotonic saline (10% body weight in 10 min) or given a control infusion (0.01 ml/min for 10 min). Ninety minutes after the start of the infusion, the rats were anesthetized and perfused transcardially. Their brains were sectioned and processed for Fos, dopamine-β-hydroxylase, and oxytocin immunocytochemistry. Volume expansion plus i.p.c. saline produced a significant increase in Fos expression in the nucleus of the solitary tract, the ventrolateral medulla, the area postrema, the locus coeruleus, the paraventricular nucleus of the hypothalamus, the perinuclear zone of the supraoptic nucleus, and oxytocin neurons in the supraoptic nucleus. The i.p.c. procaine significantly blocked Fos expression produced by the volume expansion in the all of the regions examined except for the area postrema and the SON oxytocin neurons.
UR - http://www.scopus.com/inward/record.url?scp=0036213520&partnerID=8YFLogxK
U2 - 10.1006/exnr.2002.7863
DO - 10.1006/exnr.2002.7863
M3 - Article
C2 - 11922660
AN - SCOPUS:0036213520
VL - 174
SP - 181
EP - 192
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -