TY - JOUR
T1 - INTRAMOLECULAR FLUORESCENCE QUENCHING IN COVALENT ACRYLAMIDE‐INDOLE ADDUCTS
AU - EFTINK, MAURICE R.
AU - JIA, YI‐WEI ‐W
AU - GRAVES, DAVID E.
AU - WICZK, WIESLAW
AU - GRYCZYNSKI, IGNACY
AU - LAKOWICZ, JOSEPH R.
PY - 1989/6
Y1 - 1989/6
N2 - Abstract— Indole derivatives have been prepared which have a covalently linked quencher, acrylamide. One of these adducts, N‐acrylyltryptamine, has a flexible linkage and the other, N‐acrylyl‐1,2,3,4‐tetrahydropyridoindole, has a rigid bridge between indole and the quencher. The intensity decays of these adducts were obtained using multi‐frequency phase and modulation fluorometry. The fluorescence of these adducts appears to be dynamically quenched; dominant lifetimes of 64 ps and 31 ps are found for the flexible and rigid adducts. This indicates that very rapid intramolecular quenching occurs, even when the quencher and fluorophore cannot collide. Quenching in the rigid molecule probably involves electron transfer through two sigma bonds. Anisotropy decay data were also collected and rotational correlation times of 62 ps and 163 ps are reported for the flexibile and rigid adducts, respectively.
AB - Abstract— Indole derivatives have been prepared which have a covalently linked quencher, acrylamide. One of these adducts, N‐acrylyltryptamine, has a flexible linkage and the other, N‐acrylyl‐1,2,3,4‐tetrahydropyridoindole, has a rigid bridge between indole and the quencher. The intensity decays of these adducts were obtained using multi‐frequency phase and modulation fluorometry. The fluorescence of these adducts appears to be dynamically quenched; dominant lifetimes of 64 ps and 31 ps are found for the flexible and rigid adducts. This indicates that very rapid intramolecular quenching occurs, even when the quencher and fluorophore cannot collide. Quenching in the rigid molecule probably involves electron transfer through two sigma bonds. Anisotropy decay data were also collected and rotational correlation times of 62 ps and 163 ps are reported for the flexibile and rigid adducts, respectively.
UR - http://www.scopus.com/inward/record.url?scp=84986928075&partnerID=8YFLogxK
U2 - 10.1111/j.1751-1097.1989.tb05570.x
DO - 10.1111/j.1751-1097.1989.tb05570.x
M3 - Article
AN - SCOPUS:84986928075
SN - 0031-8655
VL - 49
SP - 725
EP - 729
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
IS - 6
ER -