Interleukin-8 response in cells from the human urinary tract induced by lipopolysaccharides of Proteus mirabilis O3 and O18

Milan Chromek, Dorota Stankowska, Elham Dadfar, Wieslaw Kaca, Hodjattallah Rabbani, Annelie Brauner

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8 Scopus citations


Purpose: Proteus mirabilis is a common pathogen associated mainly with complicated urinary tract infections and sometimes with septicemia. There is great serological diversity of the microorganism. While P. mirabilis O3 is commonly found in patients with infections, the serotype O18 rarely occurs. The O18 lipopolysaccharide contains a phosphocholine substitute, which makes it unique among Proteus strains. To explain different clinical significance of the strains we evaluated the biological activity of the lipopolysaccharides of P. mirabilis O3 and O18, as measured by interleukin-8 (IL-8) production. Materials and Methods: Three cell lines were used, namely uroepithelial cells, renal epithelial cells and monocytes. IL-8 production was determined on the protein and mRNA levels using enzyme-linked immunosorbent assay and real-time polymerase chain reaction, respectively, and CD14 expression on the cell surface was studied using flow cytometry. Results: Uroepithelial cells and monocytes reacted to lipopolysaccharides by higher IL-8 production compared with renal epithelial cells. Cell specific IL-8 response corresponded to the cell expression of CD14. Renal epithelial cells produced more IL-8 after stimulation with the phosphocholine rich lipopolysaccharide O18, although adding phosphocholine alone did not induce or increase IL-8 production. Conclusions: Our data suggest that different cells within the human urinary tract have different roles during urinary tract infection. The biological activity and pathogenicity of P. mirabilis lipopolysaccharides might be determined by their specific chemical structure.

Original languageEnglish
Pages (from-to)1381-1384
Number of pages4
JournalJournal of Urology
Issue number4
StatePublished - Apr 2005


  • Interleukin-8
  • Lipopolysaccharides
  • Proteus mirabilis
  • Urinary tract
  • Urinary tract infections


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