Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography

Zi Jing Lin, Ming Ren, Lin Li, Yueming Liu, Jianzhong Su, Shaohua Yang, Hanli Liu

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Diffuse optical tomography (DOT) has been used by several groups to assess cerebral hemodynamics of cerebral ischemia in humans and animals. In this study, we combined DOT with an indocyanine green (ICG)-tracking method to achieve interleaved images of cerebral hemodynamics and blood flow index (BFI) using two middle cerebral artery occlusion (MCAO) rat models. To achieve volumetric images with high-spatial resolution, we first integrated a depth compensation algorithm (DCA) with a volumetric mesh-based rat head model to generate three-dimensional (3D) DOT on a rat brain atlas. Then, the experimental DOT data from two rat models were collected using interleaved strategy for cerebral hemodynamics and BFI during and after ischemic stroke, with and without a thrombolytic therapy for the embolic MCAO model. The acquired animal data were further analyzed using the integrated rat-atlas-guided DOT method to form time-evolving 3D images of both cerebral hemodynamics and BFI. In particular, we were able to show and identify therapeutic outcomes of a thrombolytic treatment applied to the embolism-induced ischemic model. This paper demonstrates that volumetric DOT is capable of providing high-quality, interleaved images of cerebral hemodynamics and blood perfusion in small animals during and after ischemic stroke, with excellent 3D visualization and quantifications.

Original languageEnglish
Pages (from-to)566-582
Number of pages17
JournalNeuroImage
Volume85
DOIs
StatePublished - 15 Jan 2014

Fingerprint

Cerebrovascular Circulation
Optical Tomography
Hemodynamics
Stroke
Atlases
Middle Cerebral Artery Infarction
Therapeutics
Indocyanine Green
Thrombolytic Therapy
Embolism
Brain Ischemia
Perfusion
Head
Brain

Keywords

  • Depth compensation algorithm
  • Diffuse optical tomography
  • Embolism-induced ischemic model
  • Hemodynamic concentration contrast
  • Indocyanine green
  • Middle cerebra artery occlusion
  • Stroke
  • Three-dimensional reconstruction

Cite this

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title = "Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography",
abstract = "Diffuse optical tomography (DOT) has been used by several groups to assess cerebral hemodynamics of cerebral ischemia in humans and animals. In this study, we combined DOT with an indocyanine green (ICG)-tracking method to achieve interleaved images of cerebral hemodynamics and blood flow index (BFI) using two middle cerebral artery occlusion (MCAO) rat models. To achieve volumetric images with high-spatial resolution, we first integrated a depth compensation algorithm (DCA) with a volumetric mesh-based rat head model to generate three-dimensional (3D) DOT on a rat brain atlas. Then, the experimental DOT data from two rat models were collected using interleaved strategy for cerebral hemodynamics and BFI during and after ischemic stroke, with and without a thrombolytic therapy for the embolic MCAO model. The acquired animal data were further analyzed using the integrated rat-atlas-guided DOT method to form time-evolving 3D images of both cerebral hemodynamics and BFI. In particular, we were able to show and identify therapeutic outcomes of a thrombolytic treatment applied to the embolism-induced ischemic model. This paper demonstrates that volumetric DOT is capable of providing high-quality, interleaved images of cerebral hemodynamics and blood perfusion in small animals during and after ischemic stroke, with excellent 3D visualization and quantifications.",
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Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography. / Lin, Zi Jing; Ren, Ming; Li, Lin; Liu, Yueming; Su, Jianzhong; Yang, Shaohua; Liu, Hanli.

In: NeuroImage, Vol. 85, 15.01.2014, p. 566-582.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Interleaved imaging of cerebral hemodynamics and blood flow index to monitor ischemic stroke and treatment in rat by volumetric diffuse optical tomography

AU - Lin, Zi Jing

AU - Ren, Ming

AU - Li, Lin

AU - Liu, Yueming

AU - Su, Jianzhong

AU - Yang, Shaohua

AU - Liu, Hanli

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