Interaction of central Ang II and NO on the cardiac sympathetic afferent reflex in dogs

Rong Ma, Guo Qing Zhu, Wei Wang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to test the hypothesis that the central angiotensin II (Ang II) and nitric oxide (NO) systems interact to modulate the cardiac sympathetic afferent reflex (CSAR). All dogs were anesthetized with α-chloralose (100 mg/kg, iv). They were sino-aortic baroreceptor denervated and vagotomized throughout the experiment renal sympathetic nerve activity responses to cardiac sympathetic afferent stimulation and the central gain of the CSAR were measured. Three protocols were performed: (1) intracerebroventricular injection (icv, 3 μg/h or 6 μg/h) of Ang II with and without Nω-nitro-L-arginine methyl ester (L-NAME) (icv, 1 mg/kg), (2) L-NAME (icv) with and without Ang II (icv, 6 μg/h), and (3) administration of the specific neural NO synthase (nNOS) inhibitor, S-Methyl-L-thiocitrulline (MeTC) (icv, 0.1 or 1 mM, 0.5 ml in 5 min) with and without pretreatment with the angiotensin type 1 receptor antagonist, losartan (icv, 0.125 mg/kg). The primary findings were (1) Ang II alone did not significantly affect the central sensitivity of the CSAR. However, Ang II with L-NAME enhanced this reflex, (2) even though L-NAME alone augmented the CSAR, this excitatory effect was further potentiated in the presence of Ang II and (3) MeTC significantly enhanced the central sensitivity of the CSAR. However, this enhancement did not occur after pretreatment with losartan. These data suggest that Ang II interacts with NO in the brain to modulate the CSAR and that inhibition of NO is required for facilitation of the CSAR by Ang II.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalAutonomic Neuroscience: Basic and Clinical
Volume118
Issue number1-2
DOIs
StatePublished - 31 Mar 2005

Fingerprint

Angiotensin II
Reflex
Nitric Oxide
Dogs
NG-Nitroarginine Methyl Ester
Losartan
Angiotensin II Type 1 Receptor Blockers
Chloralose
Pressoreceptors
Nitric Oxide Synthase
Kidney
Injections
Brain

Keywords

  • Angiotensin II
  • Cerebroventricle
  • L-NAME
  • Losartan
  • Nitric oxide synthase
  • Renal sympathetic nerve activity
  • S-Metyl-L-thiocitrulline

Cite this

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title = "Interaction of central Ang II and NO on the cardiac sympathetic afferent reflex in dogs",
abstract = "The aim of this study was to test the hypothesis that the central angiotensin II (Ang II) and nitric oxide (NO) systems interact to modulate the cardiac sympathetic afferent reflex (CSAR). All dogs were anesthetized with α-chloralose (100 mg/kg, iv). They were sino-aortic baroreceptor denervated and vagotomized throughout the experiment renal sympathetic nerve activity responses to cardiac sympathetic afferent stimulation and the central gain of the CSAR were measured. Three protocols were performed: (1) intracerebroventricular injection (icv, 3 μg/h or 6 μg/h) of Ang II with and without Nω-nitro-L-arginine methyl ester (L-NAME) (icv, 1 mg/kg), (2) L-NAME (icv) with and without Ang II (icv, 6 μg/h), and (3) administration of the specific neural NO synthase (nNOS) inhibitor, S-Methyl-L-thiocitrulline (MeTC) (icv, 0.1 or 1 mM, 0.5 ml in 5 min) with and without pretreatment with the angiotensin type 1 receptor antagonist, losartan (icv, 0.125 mg/kg). The primary findings were (1) Ang II alone did not significantly affect the central sensitivity of the CSAR. However, Ang II with L-NAME enhanced this reflex, (2) even though L-NAME alone augmented the CSAR, this excitatory effect was further potentiated in the presence of Ang II and (3) MeTC significantly enhanced the central sensitivity of the CSAR. However, this enhancement did not occur after pretreatment with losartan. These data suggest that Ang II interacts with NO in the brain to modulate the CSAR and that inhibition of NO is required for facilitation of the CSAR by Ang II.",
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Interaction of central Ang II and NO on the cardiac sympathetic afferent reflex in dogs. / Ma, Rong; Zhu, Guo Qing; Wang, Wei.

In: Autonomic Neuroscience: Basic and Clinical, Vol. 118, No. 1-2, 31.03.2005, p. 51-60.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Interaction of central Ang II and NO on the cardiac sympathetic afferent reflex in dogs

AU - Ma, Rong

AU - Zhu, Guo Qing

AU - Wang, Wei

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