Intensity and anisotropy decays of [Leu5] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry

Joseph R. Lakowicz; Ignacy Gryczynski; Gabor Laczko; Wieslaw Wiczk

doi:10.1016/0301-4622(93)80030-M

Intensity and anisotropy decays of [Leu⁵] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry

Joseph R. Lakowicz, Ignacy Gryczynski, Gabor Laczko, Wieslaw Wiczk

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7 Scopus citations

Abstract

The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu⁵] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r₀ = 0.4 to a value of r₀ = 0.326 occurring during the first two picoseconds after excitation.

Original language	English
Pages (from-to)	33-40
Number of pages	8
Journal	Biophysical Chemistry
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/0301-4622(93)80030-M
State	Published - Jul 1993

Keywords

Anisotropy decays
Fluorescence spectroscopy
Frequency-domain fluorometry
Molecular dynamics
[Leu] enkephalin

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10.1016/0301-4622(93)80030-M

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@article{2dd3c90c78a04b45a4d14396c48b924b,

title = "Intensity and anisotropy decays of [Leu5] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry",

abstract = "The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu5] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r0 = 0.4 to a value of r0 = 0.326 occurring during the first two picoseconds after excitation.",

keywords = "Anisotropy decays, Fluorescence spectroscopy, Frequency-domain fluorometry, Molecular dynamics, [Leu] enkephalin",

author = "Lakowicz, {Joseph R.} and Ignacy Gryczynski and Gabor Laczko and Wieslaw Wiczk",

note = "Funding Information: This work was supported by grant DMB-8804931fr om the National ScienceF oundation, with supportf or instrumentatiofnr om NSF grant DlR-8710401, and NIH grant SlO RR07510. Joseph R. Lakowicz,G abor Laczko and Wieslaw Wiczk acknowledges upport from the Medical BiotechnologyC enter at the Universityo f Maryland School of Medicine.",

year = "1993",

month = jul,

doi = "10.1016/0301-4622(93)80030-M",

language = "English",

volume = "47",

pages = "33--40",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

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TY - JOUR

T1 - Intensity and anisotropy decays of [Leu5] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Gryczynski, Ignacy

AU - Laczko, Gabor

AU - Wiczk, Wieslaw

N1 - Funding Information: This work was supported by grant DMB-8804931fr om the National ScienceF oundation, with supportf or instrumentatiofnr om NSF grant DlR-8710401, and NIH grant SlO RR07510. Joseph R. Lakowicz,G abor Laczko and Wieslaw Wiczk acknowledges upport from the Medical BiotechnologyC enter at the Universityo f Maryland School of Medicine.

PY - 1993/7

Y1 - 1993/7

N2 - The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu5] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r0 = 0.4 to a value of r0 = 0.326 occurring during the first two picoseconds after excitation.

AB - The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu5] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r0 = 0.4 to a value of r0 = 0.326 occurring during the first two picoseconds after excitation.

KW - Anisotropy decays

KW - Fluorescence spectroscopy

KW - Frequency-domain fluorometry

KW - Molecular dynamics

KW - [Leu] enkephalin

UR - http://www.scopus.com/inward/record.url?scp=0027163472&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(93)80030-M

DO - 10.1016/0301-4622(93)80030-M

M3 - Article

C2 - 8364147

AN - SCOPUS:0027163472

SN - 0301-4622

VL - 47

SP - 33

EP - 40

JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 1

ER -

Intensity and anisotropy decays of [Leu⁵] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry

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Keywords

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