TY - JOUR
T1 - Influence of quorum sensing signal molecules on biofilm formation in Proteus mirabilis O18
AU - Stankowska, Dorota
AU - Czerwonka, Grzegorz
AU - Rozalska, Sylwia
AU - Grosicka, Michalina
AU - Dziadek, Jaroslaw
AU - Kaca, Wieslaw
N1 - Funding Information:
Acknowledgments The study was supported by grant N N304 044639 from the National Science Centre. Some of the experiments were run on apparatus purchased with EU grant 2.2 Innovation Industry.
PY - 2012/1
Y1 - 2012/1
N2 - The influence of basis of quorum sensing molecules on Proteus strains is much less known as compared to Pseudomonas or Escherichia. We have previously shown that a series of acylated homoserine lactones (acyl-HSL) does not influence the ureolytic, proteolytic, or hemolytic abilities, and that the swarming motility of Proteus mirabilis rods is strain specific. The aim of the presented study was to find out if the presence of a series of acyl-HSL influences biofilm formation of P. mirabilis laboratory strain belonging to O18 serogroup. This serogroup is characterized by the presence of a unique non-carbohydrate component, namely phosphocholine. Escherichia coli and P. mirabilis O18 strains used in this work contains cloned plasmids encoding fluorescent protein genes with constitutive gene expression. In mixed biofilms in stationary and continuous flow conditions, P. mirabilis O18 overgrow whole culture. P. mirabilis O18 strain has genetically proved a presence of AI-2 quorum sensing system. Differences in biofilm structure were observed depending on the biofilm type and culture methods. From tested acylated homoserine lactones (BHL, HHL, OHL, DHL, dDHL, tDHL), a significant influence had BHL on thickness, structure, and the amount of exopolysaccharides produced by biofilms formed by P. mirabilis O18 pDsRed 2.
AB - The influence of basis of quorum sensing molecules on Proteus strains is much less known as compared to Pseudomonas or Escherichia. We have previously shown that a series of acylated homoserine lactones (acyl-HSL) does not influence the ureolytic, proteolytic, or hemolytic abilities, and that the swarming motility of Proteus mirabilis rods is strain specific. The aim of the presented study was to find out if the presence of a series of acyl-HSL influences biofilm formation of P. mirabilis laboratory strain belonging to O18 serogroup. This serogroup is characterized by the presence of a unique non-carbohydrate component, namely phosphocholine. Escherichia coli and P. mirabilis O18 strains used in this work contains cloned plasmids encoding fluorescent protein genes with constitutive gene expression. In mixed biofilms in stationary and continuous flow conditions, P. mirabilis O18 overgrow whole culture. P. mirabilis O18 strain has genetically proved a presence of AI-2 quorum sensing system. Differences in biofilm structure were observed depending on the biofilm type and culture methods. From tested acylated homoserine lactones (BHL, HHL, OHL, DHL, dDHL, tDHL), a significant influence had BHL on thickness, structure, and the amount of exopolysaccharides produced by biofilms formed by P. mirabilis O18 pDsRed 2.
UR - http://www.scopus.com/inward/record.url?scp=84857995300&partnerID=8YFLogxK
U2 - 10.1007/s12223-011-0091-4
DO - 10.1007/s12223-011-0091-4
M3 - Article
C2 - 22198843
AN - SCOPUS:84857995300
SN - 0015-5632
VL - 57
SP - 53
EP - 60
JO - Folia Microbiologica
JF - Folia Microbiologica
IS - 1
ER -