Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz, Wieslaw Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.

Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalBiophysical Chemistry
Volume38
Issue number1-2
DOIs
StatePublished - Oct 1990

Keywords

  • Diffusion
  • Distance distribution
  • Energy transfer
  • Fluorescence spectroscopy
  • Frequency-domain fluorometry
  • Molecular dynamics

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