Abstract
We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.
Original language | English |
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Pages (from-to) | 99-109 |
Number of pages | 11 |
Journal | Biophysical Chemistry |
Volume | 38 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 1990 |
Keywords
- Diffusion
- Distance distribution
- Energy transfer
- Fluorescence spectroscopy
- Frequency-domain fluorometry
- Molecular dynamics