Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz; Wieslaw Wiczk; Ignacy Gryczynski; Henryk Szmacinski; Michael L. Johnson

doi:10.1016/0301-4622(90)80044-8

Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz, Wieslaw Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.

Original language	English
Pages (from-to)	99-109
Number of pages	11
Journal	Biophysical Chemistry
Volume	38
Issue number	1-2
DOIs	https://doi.org/10.1016/0301-4622(90)80044-8
State	Published - Oct 1990

Keywords

Diffusion
Distance distribution
Energy transfer
Fluorescence spectroscopy
Frequency-domain fluorometry
Molecular dynamics

Access to Document

10.1016/0301-4622(90)80044-8

Cite this

@article{aff1e108f8f54dd184a914e7c77d53fb,

title = "Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry",

abstract = "We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.",

keywords = "Diffusion, Distance distribution, Energy transfer, Fluorescence spectroscopy, Frequency-domain fluorometry, Molecular dynamics",

author = "Lakowicz, {Joseph R.} and Wieslaw Wiczk and Ignacy Gryczynski and Henryk Szmacinski and Johnson, {Michael L.}",

note = "Funding Information: This work supported by grants DMB-8804913 and DMB-8502835 from the National Science Foundation, and grants GM-35154 and GM39617 from the National Institutes of Health. J.R.L. and W.W. acknowledge support from the Medical Biotechnology Center at the University of Maryland.",

year = "1990",

month = oct,

doi = "10.1016/0301-4622(90)80044-8",

language = "English",

volume = "38",

pages = "99--109",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

AU - Lakowicz, Joseph R.

AU - Wiczk, Wieslaw

AU - Gryczynski, Ignacy

AU - Szmacinski, Henryk

AU - Johnson, Michael L.

N1 - Funding Information: This work supported by grants DMB-8804913 and DMB-8502835 from the National Science Foundation, and grants GM-35154 and GM39617 from the National Institutes of Health. J.R.L. and W.W. acknowledge support from the Medical Biotechnology Center at the University of Maryland.

PY - 1990/10

Y1 - 1990/10

N2 - We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.

AB - We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities from 0.6 to 200 cP were obtained using propylene glycol at different temperatures (0-80° C) and methanol at 20° C. The intensity decays of naphthalene were measured in the frequency domain. Several theoretical models, including distance distributions, were used to fit the data. The results indicate that end-to-end diffusion of flexible donor-acceptor pairs can be detected and quantified using frequency-domain fluorometry, even in the presence of a distribution of donor-to-acceptor distances.

KW - Diffusion

KW - Distance distribution

KW - Energy transfer

KW - Fluorescence spectroscopy

KW - Frequency-domain fluorometry

KW - Molecular dynamics

UR - http://www.scopus.com/inward/record.url?scp=0025130577&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(90)80044-8

DO - 10.1016/0301-4622(90)80044-8

M3 - Article

C2 - 2085654

AN - SCOPUS:0025130577

SN - 0301-4622

VL - 38

SP - 99

EP - 109

JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 1-2

ER -

Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this