Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry

Joseph R. Lakowicz, Wieslaw Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities of 0.6 - 200cP were obtained using propylene glycol at different temperatures (0-80°C) and methanol at 20°C. The intensity decays of naphthalene were measured in the frequency-domain. Several theoretical models, including distance distributions were used to fit the data. The results indicate that end-to-end diffusion of flexible donor - acceptor pairs can be readily detected and quantified using frequency-domain fluorometry.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages554-569
Number of pages16
ISBN (Print)0819402451, 9780819402455
DOIs
StatePublished - 1990
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: 15 Jan 199017 Jan 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1204 pt 2
ISSN (Print)0277-786X

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period15/01/9017/01/90

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    Lakowicz, J. R., Wiczk, W., Gryczynski, I., Szmacinski, H., & Johnson, M. L. (1990). Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 554-569). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1204 pt 2). Publ by Int Soc for Optical Engineering. https://doi.org/10.1117/12.17748