Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3’-kinase/Akt signaling by global cerebral ischemia in the rat

K. L. Jin, X. O. Mao, T. Nagayama, P. C. Goldsmith, D. A. Greenberg

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Vascular endothelial growth factor is an angiogenic peptide that binds to tyrosine kinase receptors on target cells to activate signal transduction pathways involving phosphatidylinositol 3’-kinase and the serine-threonine protein kinase, Akt. To determine whether this signaling pathway is activated in cerebral ischemia, we examined the expression of vascular endothelial growth factor receptors 1 and 2, and phosphatidylinositol 3’-kinase-activated phospho-Akt, in the cerebral cortex and hippocampus following transient global cerebral ischemia in the rat. Western blot analysis and immunocytochemistry demonstrated induction of vascular endothelial growth factor receptor 1 and 2 expression, and of anti-phosphatidylinositol 3’-kinase-immunoprecipitated phospho-Akt, in vulnerable regions of the cortex and hippocampus after 15min of global ischemia and 4-72h of reperfusion.These findings demonstrate that vascular endothelial growth factor receptors and receptor-coupled signal transduction pathways are induced in ischemic brain in vivo, and could therefore participate in endogenous neuroprotective responses to ischemia.

Original languageEnglish
Pages (from-to)713-717
Number of pages5
JournalNeuroscience
Volume100
Issue number4
DOIs
StatePublished - 11 Oct 2000

Keywords

  • Angiogenesis
  • Cerebral cortex
  • Hippocampus
  • Hypoxia
  • Signal transduction
  • Stroke

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