Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3’-kinase/Akt signaling by global cerebral ischemia in the rat

K. L. Jin, X. O. Mao, T. Nagayama, P. C. Goldsmith, D. A. Greenberg

Research output: Contribution to journalArticle

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Abstract

Vascular endothelial growth factor is an angiogenic peptide that binds to tyrosine kinase receptors on target cells to activate signal transduction pathways involving phosphatidylinositol 3’-kinase and the serine-threonine protein kinase, Akt. To determine whether this signaling pathway is activated in cerebral ischemia, we examined the expression of vascular endothelial growth factor receptors 1 and 2, and phosphatidylinositol 3’-kinase-activated phospho-Akt, in the cerebral cortex and hippocampus following transient global cerebral ischemia in the rat. Western blot analysis and immunocytochemistry demonstrated induction of vascular endothelial growth factor receptor 1 and 2 expression, and of anti-phosphatidylinositol 3’-kinase-immunoprecipitated phospho-Akt, in vulnerable regions of the cortex and hippocampus after 15min of global ischemia and 4-72h of reperfusion.These findings demonstrate that vascular endothelial growth factor receptors and receptor-coupled signal transduction pathways are induced in ischemic brain in vivo, and could therefore participate in endogenous neuroprotective responses to ischemia.

Original languageEnglish
Pages (from-to)713-717
Number of pages5
JournalNeuroscience
Volume100
Issue number4
DOIs
StatePublished - 11 Oct 2000

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Phosphatidylinositol 3-Kinase
Vascular Endothelial Growth Factor Receptor
Brain Ischemia
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Signal Transduction
Hippocampus
Ischemia
Angiogenic Proteins
Protein-Serine-Threonine Kinases
Transient Ischemic Attack
Receptor Protein-Tyrosine Kinases
Cerebral Cortex
Vascular Endothelial Growth Factor A
Reperfusion
Western Blotting
Immunohistochemistry
Brain

Keywords

  • Angiogenesis
  • Cerebral cortex
  • Hippocampus
  • Hypoxia
  • Signal transduction
  • Stroke

Cite this

Jin, K. L. ; Mao, X. O. ; Nagayama, T. ; Goldsmith, P. C. ; Greenberg, D. A. / Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3’-kinase/Akt signaling by global cerebral ischemia in the rat. In: Neuroscience. 2000 ; Vol. 100, No. 4. pp. 713-717.
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Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3’-kinase/Akt signaling by global cerebral ischemia in the rat. / Jin, K. L.; Mao, X. O.; Nagayama, T.; Goldsmith, P. C.; Greenberg, D. A.

In: Neuroscience, Vol. 100, No. 4, 11.10.2000, p. 713-717.

Research output: Contribution to journalArticle

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