@article{0e4b0fe6af164ad4bfca6f05db9d7ba9,
title = "Induced pluripotent stem cells from highly endangered species",
abstract = "For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent extinction. We report generation of induced pluripotent stem cells (iPSCs) from two endangered species: a primate, the drill, Mandrillus leucophaeus and the nearly extinct northern white rhinoceros, Ceratotherium simum cottoni. iPSCs may eventually facilitate reintroduction of genetic material into breeding populations.",
author = "{Friedrich Ben-Nun}, Inbar and Montague, {Susanne C.} and Houck, {Marlys L.} and Tran, {Ha T.} and Ibon Garitaonandia and Leonardo, {Trevor R.} and Wang, {Yu Chieh} and Charter, {Suellen J.} and Laurent, {Louise C.} and Ryder, {Oliver A.} and Loring, {Jeanne F.}",
note = "Funding Information: cells were established using tissue dissociation with 0.5% (wt/vol) collagenase on a 4-mm skin biopsy. Fibroblast cultures at passage 2 were cryopreserved and accessioned into the Frozen Zoo at the Zoological Society of San Diego. Cell line 7078 was initiated on 25 February 1994 from a 15-year-old male drill, M. leucophaeus. This drill named Loon was born on 16 September 1979 and died on 17 June 2003. The fibroblast cell line was submitted to the Integrated Primate Biomaterials and Information Resource (7078-3207; PR#325) supported by a US National Science Foundation grant (BCS0094993). Cell line 17626 was initiated in January 2010, from a 10-year-old female northern white rhinoceros, C. simum cottoni. This northern white rhinoceros named Fatu was born 29 June 2000 and is alive as of 29 August 2011. Funding Information: I.F.B.-N. was supported by an unrestricted gift from the Esther O{\textquoteright}Keefe Foundation, and S.C.M. was supported by an internship from the California Institute for Regenerative Medicine (TB1-01186). The research was supported by the O{\textquoteright}Keefe Foundation and the Millipore Foundation. We thank our collaborator, M. Parast, for the histopathological examination of the teratomas, I. Slavin, X. Liao and other members of the Loring lab for helpful discussions and assistance, and G. Ben-Nun and D. Barker for support.",
year = "2011",
month = oct,
doi = "10.1038/nmeth.1706",
language = "English",
volume = "8",
pages = "829--831",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "10",
}