TY - JOUR
T1 - Indoor air pollution from solid fuels and peripheral Blood DNA methylation
T2 - Findings from a population study in Warsaw, Poland
AU - Tao, Meng Hua
AU - Zhou, Jiachen
AU - Rialdi, Alexander P.
AU - Martinez, Regina
AU - Dabek, Joanna
AU - Scelo, Ghislaine
AU - Lissowska, Jolanta
AU - Chen, Jia
AU - Boffetta, Paolo
N1 - Funding Information:
This work was supported by Mount Sinai Children׳s Environmental Health Center (CEHC Pilot Grant Program 0285-7451 ) and the European Commission׳s Inco-Copernicus Programme (Contract IC15-CT96-0313 ). In Warsaw, the study was supported by a grant from the Polish State Committee for Scientific Research (Grant SPUB-M-COPERNICUS/P-05/DZ-30/99/2000 ). Regina Martinez worked on this study under Mount Sinai Undergraduate Research Scholarship.
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - DNA methylation is a potential mechanism linking indoor air pollution to adverse health effects. Fetal and early-life environmental exposures have been associated with altered DNA methylation and play a critical role in progress of diseases in adulthood. We investigated whether exposure to indoor air pollution from solid fuels at different lifetime periods was associated with global DNA methylation and methylation at the IFG2/H19 imprinting control region (ICR) in a population-based sample of non-smoking women from Warsaw, Poland. Global methylation and IFG2/H19 ICR methylation were assessed in peripheral blood DNA from 42 non-smoking women with Luminometric Methylation Assay (LUMA) and quantitative pyrosequencing, respectively. Linear regression models were applied to estimate associations between indoor air pollution and DNA methylation in the blood. Compared to women without exposure, the levels of LUMA methylation for women who had ever exposed to both coal and wood were reduced 6.70% (95% CI: -13.36, -0.04). Using both coal and wood before age 20 was associated with 6.95% decreased LUMA methylation (95% CI: -13.79, -0.11). Further, the negative correlations were more significant with exposure to solid fuels for cooking before age 20. There were no clear associations between indoor solid fuels exposure before age 20 and through the lifetime and IFG2/H19 ICR methylation. Our study of non-smoking women supports the hypothesis that exposure to indoor air pollution from solid fuels, even early-life exposure, has the capacity to modify DNA methylation that can be detected in peripheral blood.
AB - DNA methylation is a potential mechanism linking indoor air pollution to adverse health effects. Fetal and early-life environmental exposures have been associated with altered DNA methylation and play a critical role in progress of diseases in adulthood. We investigated whether exposure to indoor air pollution from solid fuels at different lifetime periods was associated with global DNA methylation and methylation at the IFG2/H19 imprinting control region (ICR) in a population-based sample of non-smoking women from Warsaw, Poland. Global methylation and IFG2/H19 ICR methylation were assessed in peripheral blood DNA from 42 non-smoking women with Luminometric Methylation Assay (LUMA) and quantitative pyrosequencing, respectively. Linear regression models were applied to estimate associations between indoor air pollution and DNA methylation in the blood. Compared to women without exposure, the levels of LUMA methylation for women who had ever exposed to both coal and wood were reduced 6.70% (95% CI: -13.36, -0.04). Using both coal and wood before age 20 was associated with 6.95% decreased LUMA methylation (95% CI: -13.79, -0.11). Further, the negative correlations were more significant with exposure to solid fuels for cooking before age 20. There were no clear associations between indoor solid fuels exposure before age 20 and through the lifetime and IFG2/H19 ICR methylation. Our study of non-smoking women supports the hypothesis that exposure to indoor air pollution from solid fuels, even early-life exposure, has the capacity to modify DNA methylation that can be detected in peripheral blood.
KW - Epidemiology
KW - Gene-specific methylation
KW - Global methylation
KW - IGF2/H19
KW - Indoor air pollution
UR - http://www.scopus.com/inward/record.url?scp=84907457537&partnerID=8YFLogxK
U2 - 10.1016/j.envres.2014.08.017
DO - 10.1016/j.envres.2014.08.017
M3 - Article
C2 - 25199973
AN - SCOPUS:84907457537
SN - 0013-9351
VL - 134
SP - 325
EP - 330
JO - Environmental Research
JF - Environmental Research
ER -