Increasing the sensitivity of protein detection of a silver stain for agarose gels

Research output: Contribution to journalArticle

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Abstract

A silver staining method is described for increasing the sensitivity of protein detection in agarose gels. Following electrophoresis, the gels are first stained with Coomassie Brilliant Blue R‐250, then washed with glutaraldehyde and finally silver stained. Using this double‐stain method, protein detection was 100 times more sensitive than Coomassie Blue staining alone and 10 times more sensitive than when silver staining was used alone.

Original languageEnglish
Pages (from-to)174-175
Number of pages2
JournalELECTROPHORESIS
Volume5
Issue number3
DOIs
StatePublished - 1 Jan 1984

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Silver Staining
Silver
Sepharose
Coloring Agents
Gels
Glutaral
Electrophoresis
Proteins
Staining and Labeling
coomassie Brilliant Blue
Coomassie blue

Cite this

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abstract = "A silver staining method is described for increasing the sensitivity of protein detection in agarose gels. Following electrophoresis, the gels are first stained with Coomassie Brilliant Blue R‐250, then washed with glutaraldehyde and finally silver stained. Using this double‐stain method, protein detection was 100 times more sensitive than Coomassie Blue staining alone and 10 times more sensitive than when silver staining was used alone.",
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Increasing the sensitivity of protein detection of a silver stain for agarose gels. / Budowle, Bruce.

In: ELECTROPHORESIS, Vol. 5, No. 3, 01.01.1984, p. 174-175.

Research output: Contribution to journalArticle

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