Increasing the sensitivity of detection of the electrophoretic marker group specific component in agarose gels by double-staining with Coomassie Brilliant Blue R250 and silver

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Abstract

A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.

Original languageEnglish
Pages (from-to)1183-1186
Number of pages4
JournalJournal of forensic sciences
Volume29
Issue number4
StatePublished - 1 Jan 1984

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Silver
Sepharose
Gels
Staining and Labeling
Agar Gel Electrophoresis
Coloring Agents
coomassie Brilliant Blue

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title = "Increasing the sensitivity of detection of the electrophoretic marker group specific component in agarose gels by double-staining with Coomassie Brilliant Blue R250 and silver",
abstract = "A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.",
author = "Bruce Budowle",
year = "1984",
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language = "English",
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journal = "Journal of forensic sciences",
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AU - Budowle, Bruce

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N2 - A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.

AB - A method is described that increases the sensitivity of detection of group-specific component (Gc) in bloodstains following agarose gel electrophoresis. Gels are stained first with Coomassie Brilliant Blue R250 (CB) and subsequently stained with silver. Double-staining with CB first enhances the sensitivity of the silver stain from 10- to 80-fold over staining with CB alone. The method is reproducible and can easily be incorporated into the laboratory as a routine procedure.

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