Increased tissue neutral endopeptidase 24.11 Activity in spontaneously hypertensive hamsters

Jamboor K. Vishwanatha, Randall G. Davis, Shmaryahu Blumberg, Xiao Pei Gao, Israel Rubinstein

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The purpose of this study was to determine whether tissue neutral endopeptidase (NEP) 24.11 activity, a membrane-bound metalloenzyme widely distributed in the peripheral circulation that cleaves and inactivates vasodilator peptides, is increased in spontaneously hypertensive hamsters relative to genetically/age-matched normotensive hamsters. Mean arterial pressure and heart rate were 163 ± 11 mm Hg and 312 ± 7 beats/min in spontaneously hypertensive hamsters and 99 ± 3 mm Hg and 302 ± 10 beats/min in normotensive hamsters, respectively (mean ± SEM). NEP 24.11 activity is significantly increased in the kidney, cheek pouch, and spinotrapezius muscle, and significantly decreased in the heart and aorta of spontaneously hypertensive hamsters relative to controls (P < .05). Lung and brain NEP 24.11 activity is similar in both groups. Renal NEP 24.11 activity increases and to a similar extent in spontaneously hypertensive and normotensive hamsters as chloride anion concentration in the assay buffer is increased. Substituting citrate for chloride anion significantly attenuates renal NEP 24.11 activity. Taken together, these data indicate that NEP 24.11 activity in spontaneously hypertensive hamsters is increased in two organs that contribute appreciably to peripheral vascular resistance, skeletal muscle, and kidney. We suggest that the spontaneously hypertensive hamster is a suitable model to study the role of skeletal muscle and renal NEP 24.11 in regulating vasomotor tone in essential hypertension.

Original languageEnglish
Pages (from-to)585-590
Number of pages6
JournalAmerican journal of hypertension
Volume11
Issue number5
DOIs
StatePublished - May 1998

Keywords

  • Chloride
  • Genetic hypertension
  • Kidney
  • Microcirculation
  • Peptidases
  • Proteinase inhibitors
  • Skeletal muscle

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