Abstract
Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24h after 20min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24h after 90min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay.Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. Copyright (C) 1999 IBRO.
| Original language | English |
|---|---|
| Pages (from-to) | 161-168 |
| Number of pages | 8 |
| Journal | Neuroscience |
| Volume | 96 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 2000 |
Fingerprint
Keywords
- Apoptosis
- Apoptosis-linked gene 2
- Cerebral ischemia
- Gene expression
- Programmed cell death
Cite this
}
Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia. / Li, W.; Jin, K.; Nagayama, T.; He, X.; Chang, J.; Minami, M.; Graham, S. H.; Simon, R. P.; Greenberg, D. A.
In: Neuroscience, Vol. 96, No. 1, 01.01.2000, p. 161-168.Research output: Contribution to journal › Article
TY - JOUR
T1 - Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia
AU - Li, W.
AU - Jin, K.
AU - Nagayama, T.
AU - He, X.
AU - Chang, J.
AU - Minami, M.
AU - Graham, S. H.
AU - Simon, R. P.
AU - Greenberg, D. A.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24h after 20min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24h after 90min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay.Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. Copyright (C) 1999 IBRO.
AB - Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24h after 20min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24h after 90min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay.Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. Copyright (C) 1999 IBRO.
KW - Apoptosis
KW - Apoptosis-linked gene 2
KW - Cerebral ischemia
KW - Gene expression
KW - Programmed cell death
UR - http://www.scopus.com/inward/record.url?scp=0033981122&partnerID=8YFLogxK
U2 - 10.1016/S0306-4522(99)00531-X
DO - 10.1016/S0306-4522(99)00531-X
M3 - Article
C2 - 10683420
AN - SCOPUS:0033981122
VL - 96
SP - 161
EP - 168
JO - Neuroscience
JF - Neuroscience
SN - 0306-4522
IS - 1
ER -