Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia

W. Li, K. Jin, T. Nagayama, X. He, J. Chang, M. Minami, S. H. Graham, R. P. Simon, D. A. Greenberg

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Abstract

Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24h after 20min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24h after 90min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay.Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. Copyright (C) 1999 IBRO.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalNeuroscience
Volume96
Issue number1
DOIs
StatePublished - 1 Jan 2000

Keywords

  • Apoptosis
  • Apoptosis-linked gene 2
  • Cerebral ischemia
  • Gene expression
  • Programmed cell death

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    Li, W., Jin, K., Nagayama, T., He, X., Chang, J., Minami, M., Graham, S. H., Simon, R. P., & Greenberg, D. A. (2000). Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia. Neuroscience, 96(1), 161-168. https://doi.org/10.1016/S0306-4522(99)00531-X