Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia

W. Li, K. Jin, T. Nagayama, X. He, J. Chang, M. Minami, S. H. Graham, R. P. Simon, D. A. Greenberg

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Calcium is an important mediator of programmed cell death induced by transient cerebral ischemia, and calcium-binding proteins have been implicated in calcium-regulated signal transduction. Apoptosis-linked gene 2 is a calcium-binding protein required for cell death induced by different apoptotic stimuli. By Western blot analysis, we found that apoptosis-linked gene 2 protein was expressed in normal brains, and that expression increased in ischemic brains after 20 or 90min of transient focal cerebral ischemia. Immunocytochemistry showed increased apoptosis-linked gene 2 protein expression in frontal cortex, a region where neurons underwent ischemic stress but still survived, after 20 or 90min of focal cerebral ischemia. Apoptosis-linked gene 2 protein was also up-regulated in the ischemic border-zone of parietal cortex 24h after 20min of focal ischemia, and was remarkably over-expressed in the caudate-putamen and parietal cortex, (where cells are destined to die) 24h after 90min of ischemia. The expression pattern of apoptosis-linked gene 2 protein was similar to that of deoxyribonucleic acid damage detected by Klenow labeling assay.Our results suggest that apoptosis-linked gene 2 may be involved in the regulation of cell death after transient focal cerebral ischemia. Copyright (C) 1999 IBRO.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
Issue number1
StatePublished - Jan 2000


  • Apoptosis
  • Apoptosis-linked gene 2
  • Cerebral ischemia
  • Gene expression
  • Programmed cell death


Dive into the research topics of 'Increased expression of apoptosis-linked gene 2 (ALG2) in the rat brain after temporary focal cerebral ischemia'. Together they form a unique fingerprint.

Cite this