Increased expression of a 58-kDa protein kinase leads to changes in the CHO cell cycle

B. A. Bunnell, L. S. Heath, D. E. Adams, J. M. Lahti, V. J. Kidd

Research output: Contribution to journalArticle

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Abstract

We have isolated and characterized cDNA encoding a human 58-kDa protein kinase that is homologous to the cell division control (CDC) protein kinases. This protein kinase also contains a unique N-terminal domain that may potentially regulate its function. Due to its relatedness to p34(CDC2), the human p58 cDNA was overexpressed in CHO cells to determine the effect on the cell cycle. Elevated expression of p58 in these cells resulted in prolonged late telophase and early G1 phase of the cell cycle. These p58 overexpressors showed a significantly increased frequency of tubulin midbodies as well as significant increases in mitotic abnormalities. Thus, proper regulation of p58 protein kinase is essential for normal cell cycle progression in these cells.

Original languageEnglish
Pages (from-to)7467-7471
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number19
DOIs
StatePublished - 1990

Keywords

  • cell division control
  • checkpoints
  • Chinese hamster ovary cells
  • late telophase
  • tubulin midbody

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