In vivo survivors of transformed mouse ovarian surface epithelial cells display diverse phenotypes for gene expression and tumorigenicity

Xiao Lin Li, Dong Qing Zhang, David Wang, David S. Knight, Lija Yin, Jianxiong Bao, Yong Yu Liu, Jonathan Glass, James Michael Mathis, Qian Jin Zhang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ovarian cancer is the fifth most common cause of cancer death in women. Due to a lack of appropriate animal models, studies involving tumorigenicity, tumor progression and immune response at the molecular level are limited. We isolated many clones derived from thesurvivors of a transformed mouse ovarian epithelial cell line IG-10 in immune- competent mice and found that the clones displayed diverse phenotypes. Most clones were deficient in components of the MHC-I antigen presentation pathway. Soft-agarose colony assays showed different growth rates among clones. However, this did not completely correlate with each clone's in vivo tumorigenicity regarding growth, tumor mass and ascites formation, suggesting the possibility that the clones may display contrasting intrinsic gene expression. We therefore performed two types of arrays to evaluate gene expression at transcriptional and translational levels. The results showed differences in expression of COL4α5, NOS-2, and SOCS-1 genes at the transcriptional level, MIP-2 gene at the protein level and CCL5, CXCL-10, IL-1α genes at both transcriptional and protein levels between low and high tumorigenic clones. Thus, our animal cell model together with the identified genes may provide a useful tool to study ovarian cancer immune response, tumorigenicity and tumor-host cell interactions in the tumor microenvironment.

Original languageEnglish
Pages (from-to)359-370
Number of pages12
JournalTumor Biology
Volume29
Issue number6
DOIs
StatePublished - 1 Jan 2009

Fingerprint

Survivors
Clone Cells
Epithelial Cells
Phenotype
Gene Expression
Ovarian Neoplasms
Neoplasms
Animal Models
Genes
Tumor Microenvironment
Antigen Presentation
Growth
Interleukin-1
Ascites
Cell Communication
Sepharose
Cause of Death
Proteins
Cell Line

Keywords

  • Genetic alterations
  • Immune response
  • Ovarian epithelial cancer
  • Tumorigenicity

Cite this

Li, Xiao Lin ; Zhang, Dong Qing ; Wang, David ; Knight, David S. ; Yin, Lija ; Bao, Jianxiong ; Liu, Yong Yu ; Glass, Jonathan ; Mathis, James Michael ; Zhang, Qian Jin. / In vivo survivors of transformed mouse ovarian surface epithelial cells display diverse phenotypes for gene expression and tumorigenicity. In: Tumor Biology. 2009 ; Vol. 29, No. 6. pp. 359-370.
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In vivo survivors of transformed mouse ovarian surface epithelial cells display diverse phenotypes for gene expression and tumorigenicity. / Li, Xiao Lin; Zhang, Dong Qing; Wang, David; Knight, David S.; Yin, Lija; Bao, Jianxiong; Liu, Yong Yu; Glass, Jonathan; Mathis, James Michael; Zhang, Qian Jin.

In: Tumor Biology, Vol. 29, No. 6, 01.01.2009, p. 359-370.

Research output: Contribution to journalArticle

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