In vivo imaging and biodistribution of near infrared dye loaded brain-metastatic-breast-cancer-cell-membrane coated polymeric nanoparticles

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Brain metastatic breast cancer is challenging to treat due to the presence of the blood-brain barrier (BBB) and a lack of ability to target precisely. Most drugs fail to cross the BBB limiting their effectiveness. To combat this problem, a brain metastatic breast cancer cell (MDA-MB-831) membrane-coated polymeric nanoparticle (CCNP) was synthesized. The small size (∼70 nm) and anionic surface charge (-20 mV) achieved during formulation allowed for high penetration and retention in the brain when compared to the PEGylated polymeric nanoparticle alone (mPEG-PLGA or NP). Doxorubicin-loaded CCNP showed high preferential cytotoxicity in vitro. Live (4-120 h) and ex vivo near-infrared imaging in nude mice showed extended circulation and retention of CCNP compared to uncoated nanoparticles. These data indicate that drug/dye-loaded CCNPs demonstrate excellent potential for cancer theranostics of brain metastatic breast tumors.

Original languageEnglish
Article number265101
JournalNanotechnology
Volume30
Issue number26
DOIs
StatePublished - 15 Apr 2019

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Cell membranes
Brain
Coloring Agents
Dyes
Nanoparticles
Infrared radiation
Imaging techniques
Infrared imaging
Cytotoxicity
Surface charge
Pharmaceutical Preparations
Doxorubicin
Tumors
Cells
Membranes
Blood-Brain Barrier

Keywords

  • brain
  • breast cancer
  • cancer membrane
  • nanoparticles
  • near infrared

Cite this

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title = "In vivo imaging and biodistribution of near infrared dye loaded brain-metastatic-breast-cancer-cell-membrane coated polymeric nanoparticles",
abstract = "Brain metastatic breast cancer is challenging to treat due to the presence of the blood-brain barrier (BBB) and a lack of ability to target precisely. Most drugs fail to cross the BBB limiting their effectiveness. To combat this problem, a brain metastatic breast cancer cell (MDA-MB-831) membrane-coated polymeric nanoparticle (CCNP) was synthesized. The small size (∼70 nm) and anionic surface charge (-20 mV) achieved during formulation allowed for high penetration and retention in the brain when compared to the PEGylated polymeric nanoparticle alone (mPEG-PLGA or NP). Doxorubicin-loaded CCNP showed high preferential cytotoxicity in vitro. Live (4-120 h) and ex vivo near-infrared imaging in nude mice showed extended circulation and retention of CCNP compared to uncoated nanoparticles. These data indicate that drug/dye-loaded CCNPs demonstrate excellent potential for cancer theranostics of brain metastatic breast tumors.",
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In vivo imaging and biodistribution of near infrared dye loaded brain-metastatic-breast-cancer-cell-membrane coated polymeric nanoparticles. / Kumar, Piyush; Treuren, Tim Van; Ranjan, Amalendu Prakash; Chaudhary, Pankaj; Vishwanatha, Jamboor K.

In: Nanotechnology, Vol. 30, No. 26, 265101, 15.04.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Kumar, Piyush

AU - Treuren, Tim Van

AU - Ranjan, Amalendu Prakash

AU - Chaudhary, Pankaj

AU - Vishwanatha, Jamboor K.

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AB - Brain metastatic breast cancer is challenging to treat due to the presence of the blood-brain barrier (BBB) and a lack of ability to target precisely. Most drugs fail to cross the BBB limiting their effectiveness. To combat this problem, a brain metastatic breast cancer cell (MDA-MB-831) membrane-coated polymeric nanoparticle (CCNP) was synthesized. The small size (∼70 nm) and anionic surface charge (-20 mV) achieved during formulation allowed for high penetration and retention in the brain when compared to the PEGylated polymeric nanoparticle alone (mPEG-PLGA or NP). Doxorubicin-loaded CCNP showed high preferential cytotoxicity in vitro. Live (4-120 h) and ex vivo near-infrared imaging in nude mice showed extended circulation and retention of CCNP compared to uncoated nanoparticles. These data indicate that drug/dye-loaded CCNPs demonstrate excellent potential for cancer theranostics of brain metastatic breast tumors.

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