Improved separation of the common transferrin variants in gels containing pH 5–7 Ampholines and HEPES

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Abstract

An ultrathin‐layer polyacrylamide gel isoelectric focusing technique is described for obtaining highly resolved transferrin (Tf) subtypes. N‐2‐hydroxyethylpiperazine‐N′‐2‐ethanesulfonic acid (HEPES) buffer was used to flatten the pH gradient in gels containing pH 5–7 Ampholines. The result was increased separation distances for the TfC subtype bands. The method is effective even with the most recent lots of pH 5–7 Ampholines which were not capable of providing an appropriate environment for effective TfC subtyping.

Original languageEnglish
Pages (from-to)210-212
Number of pages3
JournalElectrophoresis
Volume8
Issue number4
DOIs
StatePublished - 1987

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